Application | Comment | Organism |
---|---|---|
industry | the broad temperature profile of the enzyme makes it a potential candidate for its use in the pulp and paper industry | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expressed from vector pT-xyl in Escherichia coli strain BL21(DE3)CodonPlus-RIL | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2fold increase in activity with 0.1 mM CaCl2 | Bacillus subtilis | |
Cd2+ | 2fold increase in activity with 0.1 mM CdCl2 | Bacillus subtilis | |
Co2+ | 3fold increase in activity with 0.1 mM CoCl2 | Bacillus subtilis | |
Mg2+ | 2fold increase in activity with 0.1 mM CoCl2 | Bacillus subtilis | |
additional information | activity is not dependent on metal ions | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23350 | - |
sequence analysis | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | B9ZZN9 | - |
- |
Bacillus subtilis R5 | B9ZZN9 | - |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation, anion-exchange and hydrophobic interaction column chromatographies, 17.5fold purified | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | low affinity of the enzyme towards this substrate | Bacillus subtilis | ? | - |
? | |
beechwood xylan + H2O | low affinity of the enzyme towards this substrate | Bacillus subtilis R5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | activity sharply decreases below 30°C and above 60°C | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the half-life of the recombinant protein at 50°C is 65 min whereas it is approximately 25 min at 60°C. The enzyme is not stable at 70°C and it loses almost all of its activity even 5 min after incubation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | more than 50% activity retained, enzyme activity decreases sharply below pH 5.5 and above pH 9.0, negligible amount of activity at pH below 5.0 and above 10.5 | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | - |
- |
9.44 |