Literature summary for 3.2.1.8 extracted from

Dumon, C.; Varvak, A.; Wall, M.A.; Flint, J.E.; Lewis, R.J.; Lakey, J.H.; Morland, C.; Luginbuehl, P.; Healey, S.; Todaro, T.; DeSantis, G.; Sun, M.; Parra-Gessert, L.; Tan, X.; Weiner, D.P.; Gilbert, H.J.
Engineering hyperthermostability into a GH11 xylanase is mediated by subtle changes to protein structure (2008), J. Biol. Chem., 283, 22557-22564.

Cloned(Commentary)

Cloned (Comment)	Organism
amplified fragment of Xyn11 (nucleotides 64-646) cloned into the pSE420-CHis vector and transformed into Escherichia coli strain XL1-Blue MR	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild-type Xyn11 and hyperthermostable mutant Xyn11TS crystallized to a resolution of 1.8 and 1.9 A, respectively. Crystal structure of Xyn11TS is extremely similar to the wild-type. Crystals of Xyn11TS belong to space group C2, with unit cell dimensions of a=64.7 A, b=33.6 A, c=83.1 A, alpha=90.0°, beta=101.9°, gamma=90.0°, and with one molecule occupying the crystallographic asymmetric unit. Crystals of Xyn11 belong to space group P212121, with unit cell dimensions of a=36.3 A, b=63.2 A, c=75.1 A, alpha=beta=gamma=90.0°, also with one molecule occupying the crystallographic asymmetric unit	uncultured bacterium

Crystallization (Comment)

Organism

wild-type Xyn11 and hyperthermostable mutant Xyn11TS crystallized to a resolution of 1.8 and 1.9 A, respectively. Crystal structure of Xyn11TS is extremely similar to the wild-type. Crystals of Xyn11TS belong to space group C2, with unit cell dimensions of a=64.7 A, b=33.6 A, c=83.1 A, alpha=90.0°, beta=101.9°, gamma=90.0°, and with one molecule occupying the crystallographic asymmetric unit. Crystals of Xyn11 belong to space group P212121, with unit cell dimensions of a=36.3 A, b=63.2 A, c=75.1 A, alpha=beta=gamma=90.0°, also with one molecule occupying the crystallographic asymmetric unit

uncultured bacterium

Protein Variants

Protein Variants	Comment	Organism
N14H	thermostabilizing mutation, catalytic activity is broadly similar to the wild-type	uncultured bacterium
N30V	thermostabilizing mutation	uncultured bacterium
Q10S	thermostabilizing mutation	uncultured bacterium
Q34C	thermostabilizing mutation	uncultured bacterium
Q34H	thermostabilizing mutation	uncultured bacterium
Q34L	thermostabilizing mutation, catalytic activity is broadly similar to the wild-type	uncultured bacterium
S194H	thermostabilizing mutation	uncultured bacterium
S25E	thermostabilizing mutation	uncultured bacterium
S35E	thermostabilizing mutation, catalytic activity is broadly similar to the wild-type	uncultured bacterium
S71T	thermostabilizing mutation, catalytic activity is broadly similar to the wild-type	uncultured bacterium
S9P	locks the conformation of a surface loop, catalytic activity is broadly similar to the wild-type	uncultured bacterium
S9P	thermostabilizing mutation	uncultured bacterium
S9P/T13F/N14H/Y18F/Q34L/S35E/S71T	hyperthermostable mutant Xyn11TS, retains full activity after incubation at 90°C for 60 min, catalytic activity is broadly similar to the wild-type	uncultured bacterium
T13F	increases hydrophobic interactions	uncultured bacterium
T13F	thermostabilizing mutation, catalytic activity is broadly similar to the wild-type	uncultured bacterium
T13Y	thermostabilizing mutation	uncultured bacterium
T4L	thermostabilizing mutation	uncultured bacterium
Y18F	thermostabilizing mutation, catalytic activity is broadly similar to the wild-type	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	B2LWN3	-	-

Purification (Commentary)

Purification (Comment)	Organism
mutants and full-length protein Xyn11 purified by cation exchange chromatography and gel filtration, to more than 95% electrophoretic purity	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-xylotrioside + H2O	-	uncultured bacterium	?	-	?
oat-spelt xylan + H2O	-	uncultured bacterium	?	-	?

Synonyms

Synonyms	Comment	Organism
GH11 xylanase	-	uncultured bacterium
xylanase	-	uncultured bacterium
Xyn11	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	native enzyme	uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	Xyn11 in its apo form, is not subject to thermal inactivation up to 60°C	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
2.317	-	oat-spelt xylan	hyperthermostable mutant Xyn11TS	uncultured bacterium
2.583	-	oat-spelt xylan	wild-type	uncultured bacterium
2.583	-	oat-spelt xylan	mutant S71T	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	8	recombinant form of full-length Xyn11, pH optimum of 8.0 using oat-spelled xylan and 7.2 when 4-nitrophenyl-beta-D-xylotrioside is the substrate	uncultured bacterium