Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Lee, C.C.; Kibblewhite-Accinelli, R.E.; Smith, M.R.; Wagschal, K.; Orts, W.J.; Wong, D.W.
    Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme (2008), Curr. Microbiol., 57, 301-305.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ stimulates activity with increasing amounts from 5 mM (16%) to 20 mM (31%) Bacillus licheniformis
Co2+ stimulates activity from 5 mM (16%) to 10 mM (26%) Bacillus licheniformis
Cu2+ stimulates activity at 5 mM by 20% Bacillus licheniformis
Li+ stimulates activity with increasing amounts from 5 mM (25%) to 20 mM (38%) Bacillus licheniformis
Mg2+ stimulates activity with increasing amounts from 5 mM (14%) to 20 mM (57%) Bacillus licheniformis
Mn2+ stimulates activity from 5 mM (27%) to 10 mM (40%) Bacillus licheniformis
additional information Fe2+ has little effect on enzyme activity from 5 mM to 10 mM Bacillus licheniformis
Zn2+ stimulates activity at 5 mM by 25% Bacillus licheniformis

Application

Application Comment Organism
industry the MS514-Xyn11 enzyme can be useful as an additive to mixtures designed to hydrolyze biomass as well as in applications for which it is desirable to maintain cellulose structure Bacillus licheniformis

Cloned(Commentary)

Cloned (Comment) Organism
expressed from vector pET29-MS14-X11 in Escherichia coli strain BL21(DE3)pLysE Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibits activity from 10 mM (72%) to 20 mM (88%) Bacillus licheniformis
Fe2+ inhibits activity at 20 mM by 27% Bacillus licheniformis
Mn2+ inhibits activity at 20 mM by 25% Bacillus licheniformis
Zn2+ inhibits activity from 10 mM (7%) to 20 mM (21%) Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23400
-
 sequence analysis Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis B5SYI8
-
-
Bacillus licheniformis MS5-14 B5SYI8
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity by gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O highest activity against birchwood xylan Bacillus licheniformis ?
-
 ?
birchwood xylan + H2O highest activity against birchwood xylan Bacillus licheniformis MS5-14 ?
-
 ?
additional information has negligible activity against insoluble and soluble cellulose substrates (avicel and carboxymethyl cellulose, respectively) and other glucose-based substrates (laminarin and lichenan) Bacillus licheniformis ?
-
 ?
additional information has negligible activity against insoluble and soluble cellulose substrates (avicel and carboxymethyl cellulose, respectively) and other glucose-based substrates (laminarin and lichenan) Bacillus licheniformis MS5-14 ?
-
 ?
oat spelt xylan + H2O
-
 Bacillus licheniformis ?
-
 ?
oat spelt xylan + H2O
-
 Bacillus licheniformis MS5-14 ?
-
 ?

Synonyms

Synonyms Comment Organism
xylanase
-
 Bacillus licheniformis
Xyn11
-
 Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50
-
 Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 is stable at temperatures up to 50°C. At 55°C, more than half of the activity is lost, and no activity remains at 60°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
 Bacillus licheniformis