Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
SDS-PAGE | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sephadex G-100 gel filtration | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | xylan from birchwood and wheat bran insoluble xylan | Bacillus licheniformis | additional information | reaction products are xylose to xylopentaose with xylotriose as the major product | ? | |
xylobiose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
xylohexaose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
xylopentaose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
xylotetraose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
xylotriose + H2O | - |
Bacillus licheniformis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BlxA | - |
Bacillus licheniformis |
xylanase A | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the recombinant enzyme is stable below 70°C, enzyme activity is rapidly lost above 70°C, the residual activity of the recombinant BlxA is 76% at 70°C and pH 6.0 after 2 min | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | over 80% of recombinant BlxA activity is retained after treatment of the enzyme by preincubation over a pH range of 5.0-9.0 for 1 h at 25°C | Bacillus licheniformis |