Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
SDS-PAGE, Xyl I | Halalkalibacterium halodurans |
47000 | - |
SDS-PAGE, Xyl II | Halalkalibacterium halodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
- |
- |
Halalkalibacterium halodurans C-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl 650 M column chromatography, 16/10 DEAE fast-flow column chromatography, and Mono-Q HR 5/5 column chromatography | Halalkalibacterium halodurans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
crude enzyme, in 50 mM sodium phosphate buffer, pH 7.0 | Halalkalibacterium halodurans |
9.4 | - |
after 3.8fold purification, in 50 mM sodium phosphate buffer, pH 7.0, at 60°C | Halalkalibacterium halodurans |
19.8 | - |
after 7.9fold purification, in 50 mM sodium phosphate buffer, pH 7.0, at 70°C | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity toward p-nitrophenyl-beta-D-xyloside, p-nitrophenyl-beta-D-glucoside, p-nitrophenyl-alpha-L-arabinofuranoside, p-nitrophenyl-acetate, or carboxymethylcellulose | Halalkalibacterium halodurans | ? | - |
? | |
additional information | no activity toward p-nitrophenyl-beta-D-xyloside, p-nitrophenyl-beta-D-glucoside, p-nitrophenyl-alpha-L-arabinofuranoside, p-nitrophenyl-acetate, or carboxymethylcellulose | Halalkalibacterium halodurans C-1 | ? | - |
? | |
xylan + H2O | xylan from birchwood, oat spelt, and larchwood | Halalkalibacterium halodurans | additional information | a series of xylooligosaccharides larger than xylotriose are released as the major products by Xyl I | ? | |
xylan + H2O | xylan from birchwood, oat spelt, and larchwood | Halalkalibacterium halodurans C-1 | additional information | a series of xylooligosaccharides larger than xylotriose are released as the major products by Xyl I | ? | |
xylan + H2O | xylan from birchwood, oat spelt, and larchwood | Halalkalibacterium halodurans | xylobiose + xylotriose + ? | main products | ? | |
xylan + H2O | xylan from birchwood, oat spelt, and larchwood | Halalkalibacterium halodurans C-1 | xylobiose + xylotriose + ? | main products | ? |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Halalkalibacterium halodurans |
Xyl I | - |
Halalkalibacterium halodurans |
Xyl II | - |
Halalkalibacterium halodurans |
xylanase | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Halalkalibacterium halodurans |
70 | - |
- |
Halalkalibacterium halodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | stable up to 60°C, retains 30% of original activity after incubation at 70°C | Halalkalibacterium halodurans |
70 | 90 | stable up to 70°C, 2% and 16% of the original activity are retained after incubation at 80°C and 90°C, respectively | Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Halalkalibacterium halodurans |
7 | - |
- |
Halalkalibacterium halodurans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Halalkalibacterium halodurans |
6 | 9 | - |
Halalkalibacterium halodurans |