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Literature summary for 3.2.1.8 extracted from

  • Jeong, M.Y.; Kim, S.; Yun, C.W.; Choi, Y.J.; Cho, S.G.
    Engineering a de novo internal disulfide bridge to improve the thermal stability of xylanase from Bacillus stearothermophilus No. 236 (2007), J. Biotechnol., 127, 300-309.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
S100C/N150C increased thermostability of about 5°C compared to the wild type enzyme Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus No. 236
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA agarose beads Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.44
-
 purified mutant enzyme S100C/N150C Geobacillus stearothermophilus
40.58
-
 purified wild type enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
birchwood xylan + H2O
-
 Geobacillus stearothermophilus No. 236 ?
-
 ?

Synonyms

Synonyms Comment Organism
xylanase
-
 Geobacillus stearothermophilus
XynA
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus stearothermophilus