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Literature summary for 3.2.1.8 extracted from
- A thermostable alkaline active endo-beta-1-4-xylanase from Bacillus halodurans S7: Purification and characterization (2006), Enzyme Microb. Technol., 39, 1492-1498.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 9% increase of activity at 1 mM | Halalkalibacterium halodurans |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 81% residual activity at 5 mM | Halalkalibacterium halodurans | |
| Co2+ | 63% residual activity at 5 mM | Halalkalibacterium halodurans | |
| Cu2+ | 13% residual activity at 5 mM | Halalkalibacterium halodurans | |
| dithiothreitol | 90% residual activity at 5 mM | Halalkalibacterium halodurans | |
| EDTA | 61% residual activity at 5 mM | Halalkalibacterium halodurans | |
| Hg2+ | 75% residual activity at 5 mM | Halalkalibacterium halodurans | |
| Mg2+ | 90% residual activity at 5 mM | Halalkalibacterium halodurans | |
| Mn2+ | 3% residual activity at 5 mM | Halalkalibacterium halodurans | |
| additional information | not inhibited by Fe2+, Sn2+, Ni2+, and Na+ | Halalkalibacterium halodurans | |
| Ru2+ | 95% residual activity at 1 mM | Halalkalibacterium halodurans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 3% increase of activity at 1 mM | Halalkalibacterium halodurans | |
| Co2+ | 19% increase of activity at 1 mM | Halalkalibacterium halodurans | |
| Cu2+ | 7% increase of activity at 1 mM | Halalkalibacterium halodurans | |
| Fe2+ | 10% increase of activity at 1 mM | Halalkalibacterium halodurans | |
| K+ | 3% increase of activity at 5 mM | Halalkalibacterium halodurans | |
| Mg2+ | 4% increase of activity at 1 mM | Halalkalibacterium halodurans | |
| Ni2+ | 22% increase of activity at 5 mM | Halalkalibacterium halodurans | |
| Ru2+ | 5% increase of activity at 5 mM | Halalkalibacterium halodurans | |
| Sn2+ | 25% increase of activity at 5 mM | Halalkalibacterium halodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | - |
- |
- |
| Halalkalibacterium halodurans S7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sepharose Fast Flow column chromatography and Sephadex G-75 gel filtration | Halalkalibacterium halodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
culture supernatant | Halalkalibacterium halodurans |
| 342 | - |
after purification | Halalkalibacterium halodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | 95% activity compared to birchwood xylan | Halalkalibacterium halodurans | ? | - |
? | |
| beechwood xylan + H2O | 95% activity compared to birchwood xylan | Halalkalibacterium halodurans S7 | ? | - |
? | |
| birchwood xylan + H2O | 100% activity | Halalkalibacterium halodurans | ? | - |
? | |
| birchwood xylan + H2O | 100% activity | Halalkalibacterium halodurans S7 | ? | - |
? | |
| additional information | no detectable activity with carboxymethyl cellulose, avicel, laminarin, p-nitrophenyl-beta-galactopyranoside, p-nitrophenyl-alpha-glucopyranoside, p-nitrophenyl-beta-xylopyranoside, p-nitrophenyl-alpha-L-arabinofuranoside, p-nitrophenyl-acetate or p-nitrophenyl-alpha-D-xylopyranoside | Halalkalibacterium halodurans | ? | - |
? | |
| additional information | no detectable activity with carboxymethyl cellulose, avicel, laminarin, p-nitrophenyl-beta-galactopyranoside, p-nitrophenyl-alpha-glucopyranoside, p-nitrophenyl-beta-xylopyranoside, p-nitrophenyl-alpha-L-arabinofuranoside, p-nitrophenyl-acetate or p-nitrophenyl-alpha-D-xylopyranoside | Halalkalibacterium halodurans S7 | ? | - |
? | |
| oat spelt xylan + H2O | 85% activity compared to birchwood xylan | Halalkalibacterium halodurans | ? | - |
? | |
| oat spelt xylan + H2O | 85% activity compared to birchwood xylan | Halalkalibacterium halodurans S7 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
at pH 10 | Halalkalibacterium halodurans |
| 75 | - |
at pH 9 | Halalkalibacterium halodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | at 70°C | Halalkalibacterium halodurans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10.5 | fully stable from pH 5.5 to 10.5 after 12 h of incubation at 50°C | Halalkalibacterium halodurans |
| 9 | - |
the enzyme shows good thermal stability when incubated at 65°C in pH 9 buffer | Halalkalibacterium halodurans |
| 10 | - |
no detectable activity after 1 h at pH 10 at 65°C | Halalkalibacterium halodurans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Halalkalibacterium halodurans | isoelectric focusing | - |
4.5 |
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