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Literature summary for 3.2.1.8 extracted from

  • Berrin, J.G.; Ajandouz, e.l.H.; Georis, J.; Arnaut, F.; Juge, N.
    Substrate and product hydrolysis specificity in family 11 glycoside hydrolases: an analysis of Penicillium funiculosum and Penicillium griseofulvum xylanases (2007), Appl. Microbiol. Biotechnol., 74, 1001-1010.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (pLys) cells as glutathione S-transferase fusion protein Penicillium griseofulvum
expressed in Escherichia coli BL21 (pLys) cells as glutathione S-transferase fusion protein Talaromyces funiculosus

Inhibitors

Inhibitors Comment Organism Structure
additional information XynA is insensitive towards protein XIP-I Penicillium griseofulvum
protein XIP-I
-
 Talaromyces funiculosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20800
-
 SDS-PAGE Penicillium griseofulvum
20830
-
 MALDI-TOF mass spectrometry Penicillium griseofulvum
21140
-
 MALDI-TOF mass spectrometry Talaromyces funiculosus
23200
-
 SDS-PAGE Talaromyces funiculosus

Organism

Organism UniProt Comment Textmining
Penicillium griseofulvum
-
-
-
Talaromyces funiculosus Q9HFH0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration Penicillium griseofulvum
recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration Talaromyces funiculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
 Penicillium griseofulvum ?
-
 ?
birchwood xylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
low-viscosity wheat arabinoxylan + H2O
-
 Penicillium griseofulvum ?
-
 ?
low-viscosity wheat arabinoxylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
medium viscosity wheat arabinoxylan + H2O
-
 Penicillium griseofulvum ?
-
 ?
medium viscosity wheat arabinoxylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
additional information no detectable activity on xylobiose Penicillium griseofulvum ?
-
 ?
additional information no detectable activity on xylobiose Talaromyces funiculosus ?
-
 ?
wheat soluble arabinoxylan + H2O
-
 Penicillium griseofulvum xylotriose + xylobiose predominant production of xylotriose and xylobiose as end products ?
wheat soluble arabinoxylan + H2O
-
 Talaromyces funiculosus xylotriose + xylobiose predominant production of xylotriose and xylobiose as end products ?
xylohexaose + H2O rapid cleavage Penicillium griseofulvum 2 xylotriose xylohexaose is cleaved initially to produce mainly xylotriose and a small amount of xylobiose and xylotetraose ?
xylohexaose + H2O rapid cleavage Talaromyces funiculosus 2 xylotriose xylohexaose is cleaved initially to produce mainly xylotriose and a small amount of xylobiose and xylotetraose ?
xylopentaose + H2O rapid cleavage Penicillium griseofulvum xylobiose + xylotriose
-
 ?
xylopentaose + H2O rapid cleavage Talaromyces funiculosus xylobiose + xylotriose
-
 ?
xylotetraose + H2O low activity Penicillium griseofulvum 2 xylobiose
-
 ?
xylotetraose + H2O low activity Talaromyces funiculosus 2 xylobiose
-
 ?
xylotriose + H2O low activity Penicillium griseofulvum ?
-
 ?
xylotriose + H2O low activity Talaromyces funiculosus ?
-
 ?

Synonyms

Synonyms Comment Organism
xylanase
-
 Penicillium griseofulvum
xylanase
-
 Talaromyces funiculosus
XynA
-
 Penicillium griseofulvum
XynC
-
 Talaromyces funiculosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Penicillium griseofulvum
50 60
-
 Talaromyces funiculosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable at 30°C for 60 min, with more than 95% of the activity remaining Penicillium griseofulvum
30
-
 stable at 30°C for 60 min, with more than 95% of the activity remaining Talaromyces funiculosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
277
-
 birchwood xylan apparent value for the native enzyme Penicillium griseofulvum
292
-
 birchwood xylan apparent value for the recombinant enzyme Penicillium griseofulvum
872
-
 birchwood xylan apparent value for the native enzyme Talaromyces funiculosus
997
-
 birchwood xylan apparent value for the recombinant enzyme Talaromyces funiculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Penicillium griseofulvum
5.5
-
-
 Talaromyces funiculosus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 displays over 50% activity in the pH range of 4.5 to 6.5 Penicillium griseofulvum
4.5 6.5 displays over 50% activity in the pH range of 4.5 to 6.5 Talaromyces funiculosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 around 30% activity is still observed at pH 7.5 Penicillium griseofulvum
7.5
-
 no activity is observed at pH 7.5 Talaromyces funiculosus

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium griseofulvum isoelectric focusing
-
 4.2
Talaromyces funiculosus isoelectric focusing
-
 8.6