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Literature summary for 3.2.1.8 extracted from

  • Sapre, M.P.; Jha, H.; Patil, M.B.
    Purification and characterization of a thermoalkalophilic xylanase from Bacillus sp. (2005), World J. Microbiol. Biotechnol., 21, 649-654.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
glutathione 10 mM, 10% stimulation Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 6 mM, 70% inhibition Bacillus sp. (in: Bacteria)
Hg2+ strong, no reactivation by EDTA Bacillus sp. (in: Bacteria)
N-bromosuccinimide inactivation Bacillus sp. (in: Bacteria)
Pb2+ strong Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
439
-
pH 6.5, 50°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O
-
Bacillus sp. (in: Bacteria) xylobiose + xylose + ? highly specific substrate ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable for up to 72 h Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
and also pH 8.5 and 10.5 Bacillus sp. (in: Bacteria)
8.5
-
and also pH 10.5 and 6.5 Bacillus sp. (in: Bacteria)
10.5
-
and also pH 8.5 and 6.5 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10.5
-
Bacillus sp. (in: Bacteria)