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Literature summary for 3.2.1.8 extracted from
- Purification and properties of three endo-xylanases from Acremonium cellulolyticus (2001), J. Appl. Glycosci., 48, 45-54.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | all 3 isozymes | Talaromyces pinophilus |  |
| KMnO4 | almost complete inhibition of all 3 isozymes at 1 mM | Talaromyces pinophilus | |
| PCMB | all 3 isozymes | Talaromyces pinophilus | |
| SDS | all 3 isozymes | Talaromyces pinophilus | |
| Zn2+ | all 3 isozymes | Talaromyces pinophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Talaromyces pinophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25500 | - |
x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE | Talaromyces pinophilus |
| 30000 | - |
x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE | Talaromyces pinophilus |
| 33500 | - |
x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE | Talaromyces pinophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces pinophilus | - |
3 isozymes I, II, and III | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| all 3 enzymes | Talaromyces pinophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 74.8 | - |
purified isozyme III | Talaromyces pinophilus |
| 86.1 | - |
purified isozyme II | Talaromyces pinophilus |
| 112.1 | - |
purified isozyme I | Talaromyces pinophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity | Talaromyces pinophilus | ? | - |
? | |
| xylan + H2O | - |
Talaromyces pinophilus | ? | - |
? | |
| xylooligosaccharides + H2O | - |
Talaromyces pinophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE | Talaromyces pinophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase | - |
Talaromyces pinophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
all 3 isozymes | Talaromyces pinophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
isozyme III is stable below | Talaromyces pinophilus |
| 55 | - |
isozymes I and II are stable below | Talaromyces pinophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
isozymes I and III | Talaromyces pinophilus |
| 3.8 | - |
isozyme II | Talaromyces pinophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 9.5 | isozyme III, completely stable at 25°C, 2 h | Talaromyces pinophilus |
| 3 | 9.5 | isozyme II, completely stable at 25°C, 2 h | Talaromyces pinophilus |
| 3.5 | 9.5 | isozyme I, completely stable at 25°C, 2 h | Talaromyces pinophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Talaromyces pinophilus | isozyme I | - |
4.9 |
| Talaromyces pinophilus | isozyme II | - |
4.9 |
| Talaromyces pinophilus | isozyme III | - |
6 |
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