Cloned (Comment) | Organism |
---|---|
library construction, cDNA isolation, DNA and amino acid sequence determination | Carica papaya |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Carica papaya | |
Co2+ | - |
Carica papaya | |
Mg2+ | - |
Carica papaya | |
Mn2+ | - |
Carica papaya | |
additional information | no inhibition by EDTA and DTT | Carica papaya | |
SDS | strong inhibition | Carica papaya | |
Zn2+ | - |
Carica papaya |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Carica papaya | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
x * 32500, purified mature enzyme, SDS-PAGE, x * 64960, unprocessed enzyme, amino acid sequence calculation | Carica papaya |
64960 | - |
x * 32500, purified mature enzyme, SDS-PAGE, x * 64960, unprocessed enzyme, amino acid sequence calculation | Carica papaya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Carica papaya | enzyme is responsible for softening of fruits during ripening, it is involved in cell wall degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | Q8GTJ2 | enzyme CpaEXY1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme contains a predicted carbohydrate binding site | Carica papaya |
proteolytic modification | the enzyme is post-translationally modified in the cell wall serving for regulation of the enzyme activity and cell wall-microdomain-specific hydrolysis | Carica papaya |
Purification (Comment) | Organism |
---|---|
44871fold from ripe fruit mesocarp, to homogeneity | Carica papaya |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | ripe | Carica papaya | - |
mesocarp | of ripe fruit | Carica papaya | - |
additional information | developmentally expressed during fruit ripening | Carica papaya | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-beta-D-glucuronoxylan + H2O | soluble xylan, commercial substrate | Carica papaya | ? | - |
? | |
additional information | enzyme is responsible for softening of fruits during ripening, it is involved in cell wall degradation | Carica papaya | ? | - |
? | |
xylan + H2O | part of hemicelluloses in cell walls | Carica papaya | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32500, purified mature enzyme, SDS-PAGE, x * 64960, unprocessed enzyme, amino acid sequence calculation | Carica papaya |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Carica papaya |
EXY1 | - |
Carica papaya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
about | Carica papaya |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
rapid decline in activity above 45°C | Carica papaya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Carica papaya |