KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and mutant strain enzyme | Cellulomonas flavigena |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cellulomonas flavigena | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, wild-type and mutant strain enzyme, SDS-PAGE | Cellulomonas flavigena |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas flavigena | - |
and its mutant PN-120 | - |
Cellulomonas flavigena CDBB531 | - |
and its mutant PN-120 | - |
Purification (Comment) | Organism |
---|---|
wild-type, 27.5fold, and mutant strain enzyme, 30.6fold, to homogeneity from culture supernatant | Cellulomonas flavigena |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
322 | - |
purified wild-type enzyme | Cellulomonas flavigena |
2500 | - |
purified mutant enzyme | Cellulomonas flavigena |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | from birchwood | Cellulomonas flavigena | ? | - |
? | |
xylan + H2O | from birchwood | Cellulomonas flavigena CDBB531 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, wild-type and mutant strain enzyme, SDS-PAGE | Cellulomonas flavigena |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Cellulomonas flavigena |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | wild-type enzyme | Cellulomonas flavigena |
55 | - |
mutant enzyme | Cellulomonas flavigena |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
wild-type enzyme | Cellulomonas flavigena |
7 | - |
mutant enzyme | Cellulomonas flavigena |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | the mutant enzyme shows higher activity at pH 9.0 than the wild-type enzyme | Cellulomonas flavigena |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cellulomonas flavigena | isoelectric focusing, wild-type and mutant strain enzyme | - |
6 |