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Literature summary for 3.2.1.8 extracted from

  • George, S.P.; Ahmad, A.; Rao, M.B.
    Involvement of a lysine residue in the active site of a thermostable xylanase from Thermomonospora sp (2001), Biochem. Biophys. Res. Commun., 282, 48-54.
    View publication on PubMed
Search for more literature for 3.2.1.8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000 Thermomonospora sp.

Organism

Organism UniProt Comment Textmining
Thermomonospora sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
13.3fold to homogeneity Thermomonospora sp.

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m a lysine residue is involved in substrate binding and is located at the binding site Thermomonospora sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimal growth at pH 9.0 and 50°C Thermomonospora sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
455
-
 purified enzyme Thermomonospora sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O from oat spelt Thermomonospora sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000 Thermomonospora sp.
More the enzyme is composed of 38% alpha-helix and 10% beta-sheet Thermomonospora sp.

Synonyms

Synonyms Comment Organism
More enzyme belongs to family 10 Thermomonospora sp.
Xyl I
-
 Thermomonospora sp.
xylanase
-
 Thermomonospora sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermomonospora sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life 86 min Thermomonospora sp.
90
-
 half-life 30 min Thermomonospora sp.
100
-
 half-life 15 min Thermomonospora sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
223.3
-
 xylan 50°C Thermomonospora sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable, 75% residual activity Thermomonospora sp.

pI Value

Organism Comment pI Value Maximum pI Value
Thermomonospora sp. isoelectric focusing
-
 4.2