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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of two endo-1,4-beta-xylanases and a 3-xylosidase from Phlebia radiata (2001), Acta Microbiol. Pol., 50, 117-128.
Application
| Application | Comment | Organism |
|---|---|---|
| paper production | enzyme can be used in pulp-bleaching processes to remove hemicellulose and release lignin from the pulp, leading to reduction of chloride required for conventional chemical bleaching | Phlebia radiata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Phlebia radiata |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Phlebia radiata | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15800 | - |
isozyme XA-2, gel filtration | Phlebia radiata |
| 18600 | - |
isozyme XA-1, gel filtration | Phlebia radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phlebia radiata | - |
isozymes XA-1 and XA-2 | - |
| Phlebia radiata 79 / ATCC 64658 | - |
isozymes XA-1 and XA-2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content of isozyme XA-1 is 6.7% and of isozyme Xa-2 3.58% | Phlebia radiata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozymes XA-1 and XA-2 from culture medium, to homogeneity, purification of Xa-1 56.1fold, of Xa-2 91.2fold | Phlebia radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | grown on wheat bran | Phlebia radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.75 | - |
purified isozyme XA-1 | Phlebia radiata |
| 49.99 | - |
purified isozyme XA-2 | Phlebia radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no arabinose-releasing activity with arabinoxylan substrates from rye arabinoxylan and birchwood xylan | Phlebia radiata | ? | - |
? |  |
| additional information | no arabinose-releasing activity with arabinoxylan substrates from rye arabinoxylan and birchwood xylan | Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
| xylan + H2O | from larchwood, both isozymes are endo-acting | Phlebia radiata | ? | - |
? | |
| xylan + H2O | from larchwood, both isozymes are endo-acting | Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Phlebia radiata |
| Xa | - |
Phlebia radiata |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phlebia radiata | isozyme XA-2, chromatofocusing | - |
4.2 |
| Phlebia radiata | isozyme XA-1, chromatofocusing | - |
7.1 |
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