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Literature summary for 3.2.1.8 extracted from
- Nucleotide sequence of the Clostridium stercorarium xynB gene encoding an extremely thermostable xylanase and characterization of the translated product (1995), Biosci. Biotechnol. Biochem., 59, 40-46.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermoclostridium stercorarium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, SDS-PAGE | Thermoclostridium stercorarium |
| 44377 | - |
x * 44377, calculation from nucleotide sequence | Thermoclostridium stercorarium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | 32 amino acids are removed from the N-terminus of Xyn B by proteolysis, resulting in the formation of a protein species with a predicted molecular weight of 40805 Da | Thermoclostridium stercorarium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoclostridium stercorarium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | - |
Thermoclostridium stercorarium | additional information | - |
? |  |
| carboxymethylcellulose + H2O | - |
Thermoclostridium stercorarium | ? | - |
? | |
| p-nitrophenyl beta-D-cellobioside + H2O | - |
Thermoclostridium stercorarium | ? | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside | - |
Thermoclostridium stercorarium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE | Thermoclostridium stercorarium |
| ? | x * 44377, calculation from nucleotide sequence | Thermoclostridium stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
hydrolysis of xylan | Thermoclostridium stercorarium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | 60°C: about 50% of maximal activity, 90°C: about 20% of maximal activity | Thermoclostridium stercorarium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable up to | Thermoclostridium stercorarium |
| 100 | - |
10 min, retains 50% of its activity at pH 6.1, retains 58% of its activity at pH 6.4, completly inactivated at pH 5.8 | Thermoclostridium stercorarium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermoclostridium stercorarium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 80% of maximal activity, pH 8.0: about 65% of maximal activity | Thermoclostridium stercorarium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 12 | 4°C, 24 h, stable | Thermoclostridium stercorarium |
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