Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | stimulates | Bacillus amyloliquefaciens | |
dithiothreitol | stimulates | Bacillus amyloliquefaciens | |
EDTA | 1-5 mM, stimulates | Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Bacillus amyloliquefaciens | |
Fe3+ | - |
Bacillus amyloliquefaciens | |
Hg2+ | - |
Bacillus amyloliquefaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus amyloliquefaciens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | stimulates | Bacillus amyloliquefaciens | |
Mn2+ | maximal activity at 2 mM | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18500 | - |
equilibrium sedimentation | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is either a glycoprotein or possesses a tightly bound carbohydrate residue | Bacillus amyloliquefaciens |
Purification (Comment) | Organism |
---|---|
- |
Bacillus amyloliquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
213.1 | - |
- |
Bacillus amyloliquefaciens |
Subunits | Comment | Organism |
---|---|---|
More | two protein bands are detected by SDS-PAGE: 18400 Da and 19600 Da | Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 40°C: about 50% of maximal activity, 90°C: about 85% of maximal activity | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for several days | Bacillus amyloliquefaciens |
55 | - |
pH 6.8, 15 min, stable up to | Bacillus amyloliquefaciens |
75 | - |
pH 6.8, 15 min, nearly all activity is destroyed | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | - |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | about 40% of maximal activity at pH 3.0 and at pH 10.0 | Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
50°C, 5 h, stable | Bacillus amyloliquefaciens |
10 | - |
50°C, 5 h, less than 20% loss of activity | Bacillus amyloliquefaciens |