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Literature summary for 3.2.1.8 extracted from
- Purification and biochemical characteristics of beta-D-xylanase from a thermophilic fungus, Thermomyces lanuginosus - SSBP (1999), Biotechnol. Appl. Biochem., 30, 73-79.
Application
| Application | Comment | Organism |
|---|---|---|
| paper production | potential use in biopulping processes | Thermomyces lanuginosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis-(2-nitrobenzoic acid) | - |
Thermomyces lanuginosus |  |
| beta-mercaptoethanol | - |
Thermomyces lanuginosus | |
| diethyl dicarbonate | - |
Thermomyces lanuginosus | |
| dithiothreitol | - |
Thermomyces lanuginosus | |
| DTNB | - |
Thermomyces lanuginosus | |
| Hg2+ | 6 mM, complete inhibition | Thermomyces lanuginosus | |
| iodoacetamide | - |
Thermomyces lanuginosus | |
| N-bromosuccinimide | - |
Thermomyces lanuginosus | |
| Pb2+ | 8 mM, 26% loss ofv activity | Thermomyces lanuginosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
gel filtration | Thermomyces lanuginosus |
| 23600 | - |
x * 23600, SDS-PAGE | Thermomyces lanuginosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | - |
- |
- |
| Thermomyces lanuginosus SSBP | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces lanuginosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermomyces lanuginosus |
| 3209 | - |
- |
Thermomyces lanuginosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, retains full activity after 9 months | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | - |
Thermomyces lanuginosus | additional information | - |
? | |
| 1,4-beta-D-xylan + H2O | - |
Thermomyces lanuginosus SSBP | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23600, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | - |
Thermomyces lanuginosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 85 | about 50% of maximal activity at 50°C and at 85°C | Thermomyces lanuginosus |
| 70 | 75 | - |
Thermomyces lanuginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for more than 48 h | Thermomyces lanuginosus |
| 75 | - |
30 min, 80% loss of activity | Thermomyces lanuginosus |
| 90 | - |
30 min, complete inactivation | Thermomyces lanuginosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermomyces lanuginosus |
| 6.5 | 7 | - |
Thermomyces lanuginosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
stable up to | Thermomyces lanuginosus |
| 5 | 12 | stable | Thermomyces lanuginosus |
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