Literature summary for 3.2.1.8 extracted from
Ruiz, M.C.; di Pietro, A.; Roncero, M.I.G.
Purification and characterization of an acidic endo-beta-1,4-xylanase from the tomato vascular pathogen Fusarium oxysporum f. sp. lycopersici (1997), FEMS Microbiol. Lett., 148, 75-82.
No PubMed abstract available
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
Ca2+ |
10 mM, 4% inhibition |
Fusarium oxysporum |
|
Co2+ |
10 mM, 19% inhibition |
Fusarium oxysporum |
|
Cu2+ |
10 mM, 46% inhibition |
Fusarium oxysporum |
|
Fe3+ |
10 mM, 75% inhibition |
Fusarium oxysporum |
|
Hg2+ |
10 mM, 85% inhibition |
Fusarium oxysporum |
|
Zn2+ |
10 mM, 49% inhibition |
Fusarium oxysporum |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
40000 |
- |
x * 40000, SDS-PAGE |
Fusarium oxysporum |
Organism
Organism |
UniProt |
Comment |
Textmining |
Fusarium oxysporum |
- |
f. sp. lycopersici, at least one acidic and one basic xylanase |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
N-glycosylated |
Fusarium oxysporum |
Purification (Commentary)
Purification (Comment) |
Organism |
acidic endo-beta-1,4-xylanase |
Fusarium oxysporum |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
- |
Fusarium oxysporum |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
1,4-beta-D-xylan + H2O |
- |
Fusarium oxysporum |
additional information |
at early stages of hydrolysis, polysaccharides of an intermediate degree of polymerization are detected. After complete hydrolysis, xylose and xylobiose are the major products |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 40000, SDS-PAGE |
Fusarium oxysporum |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
40 |
- |
- |
Fusarium oxysporum |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
40 |
60 |
more than 80% of maximal activity at 40°C and at 60°C |
Fusarium oxysporum |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
4.5 |
- |
- |
Fusarium oxysporum |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
3.5 |
5.5 |
more than 75% of maximal activity at pH 3.5 and at pH 5.5 |
Fusarium oxysporum |