Cloned (Comment) | Organism |
---|---|
overexpression in Streptomyces lividans | Thermobifida alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, significant inhibition | Thermobifida alba | |
Cu2+ | - |
Thermobifida alba | |
Hg2+ | 10 mM, significant inhibition | Thermobifida alba | |
Zn2+ | 10 mM, significant inhibition | Thermobifida alba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermobifida alba | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida alba | - |
ULJB1 | - |
Thermobifida alba ULJB1 | - |
ULJB1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the protein contains a 38-amino-acid leader peptide with 6 Arg residues in its amino-terminal end, a catalytic domain and a cellulose-binding domain connected by a linker region rich in Pro and Gly. A 38000 Da enzyme form and a 48000 Da enzyme form are detected by SDS-PAGE. The 38000 Da enzyme form does not contain the cellulose-binding domain | Thermobifida alba |
Purification (Comment) | Organism |
---|---|
- |
Thermobifida alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-beta-D-xylan + H2O | - |
Thermobifida alba | additional information | xylobiose and traces of xylose | ? | |
1,4-beta-D-xylan + H2O | - |
Thermobifida alba ULJB1 | additional information | xylobiose and traces of xylose | ? | |
lichenan + H2O | no activity | Thermobifida alba | ? | - |
? | |
lichenan + H2O | no activity | Thermobifida alba ULJB1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | a 38000 Da enzyme form and a 48000 Da enzyme form are detected by SDS-PAGE. The 38000 Da enzyme form does not contain the cellulose-binding domain | Thermobifida alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermobifida alba |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | 50°C: about 40% of maximal activity, 100°C: about 65% of maximal activity | Thermobifida alba |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 1 h, stable | Thermobifida alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermobifida alba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | pH 4.5: about 55% of maximal activity, pH 10.0: about 60% of maximal activity | Thermobifida alba |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 1 h, stable | Thermobifida alba |