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Literature summary for 3.2.1.8 extracted from

  • Yoshioka, H.; Nagato, N.; Chavanich, S.; Nilubol, N.; Hayashida, S.
    Purification and properties of thermostable xylanase from Talaromyces byssochlamydoides YH-50 (1981), Agric. Biol. Chem., 45, 2425-2432.
No PubMed abstract available
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Rasamsonia byssochlamydoides
KMnO4
-
 Rasamsonia byssochlamydoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, xylanase X-b-II, SDS-PAGE Rasamsonia byssochlamydoides
54000
-
 x * 54000, xylanase X-b-I, SDS-PAGE Rasamsonia byssochlamydoides
76000
-
 x * 76000, xylanase X-a, SDS-PAGE Rasamsonia byssochlamydoides

Organism

Organism UniProt Comment Textmining
Rasamsonia byssochlamydoides
-
-
-
Rasamsonia byssochlamydoides YH-50
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme form X-a contains 36.6% carbohydrate, enzyme form X-b-I contains 31.5% carbohydrate, enzyme form X-a contains 14.2% carbohydrate Rasamsonia byssochlamydoides

Purification (Commentary)

Purification (Comment) Organism
enzyme form X-1, X-b-1 and X-b-II Rasamsonia byssochlamydoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
 Rasamsonia byssochlamydoides additional information hydrolysis products with enzyme X-a are xylose + arabinose + glucose + xylobiose + xylooligosaccharides. The hydrolysis products with enzyme X-b-I and X-b-II are xylose + xylobiose ?
1,4-beta-D-xylan + H2O
-
 Rasamsonia byssochlamydoides YH-50 additional information hydrolysis products with enzyme X-a are xylose + arabinose + glucose + xylobiose + xylooligosaccharides. The hydrolysis products with enzyme X-b-I and X-b-II are xylose + xylobiose ?

Subunits

Subunits Comment Organism
? x * 76000, xylanase X-a, SDS-PAGE Rasamsonia byssochlamydoides
? x * 54000, xylanase X-b-I, SDS-PAGE Rasamsonia byssochlamydoides
? x * 45000, xylanase X-b-II, SDS-PAGE Rasamsonia byssochlamydoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 xylanase X-b-I and X-b-II Rasamsonia byssochlamydoides
75
-
 xylanase X-a Rasamsonia byssochlamydoides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 pH 5.5, 5 min, xylanase X-a, X-b-I and X-b-II, stable up to Rasamsonia byssochlamydoides
95
-
 5 min, enzyme form X-a retains 65% of the original activity, enzyme form X-b-I retains 65% of the original activity, enzyme form X-b-II retains 65% of the original activity Rasamsonia byssochlamydoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 xylanase X-b-I Rasamsonia byssochlamydoides
4.5 5.5
-
 Rasamsonia byssochlamydoides
5
-
 xylanase X-b-II Rasamsonia byssochlamydoides
5.5
-
 xylanase X-a Rasamsonia byssochlamydoides

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 4°C, 24 h, xylanase X-a and X-b-II, stable Rasamsonia byssochlamydoides
3.5 8.5 4°C, 24 h, xylanase X-b-I, stable Rasamsonia byssochlamydoides