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Literature summary for 3.2.1.8 extracted from

  • Berenger, J.F.; Frixon, C.; Bigliardi, J.; Creuzet, N.
    Production, purification, and properties of thermostable xylanase from Clostridium stercorarium (1985), Can. J. Microbiol., 31, 635-643.
No PubMed abstract available
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ CaCl2 Thermoclostridium stercorarium
Cu2+ CuSO4 Thermoclostridium stercorarium
Fe3+ FeCl3 Thermoclostridium stercorarium
Hg2+
-
 Thermoclostridium stercorarium
PHMB
-
 Thermoclostridium stercorarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thermoclostridium stercorarium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 xylanase A, disc gel electrophoresis Thermoclostridium stercorarium
44000
-
 1 * 44000, xylanase A, SDS-PAGE Thermoclostridium stercorarium
44000
-
 1 * 44000, xylanase C, SDS-PAGE Thermoclostridium stercorarium
64000
-
 xylanase C, disc gel electrophoresis Thermoclostridium stercorarium
73000
-
 xylanase B, disc gel electrophoresis Thermoclostridium stercorarium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,4-beta-D-xylan + H2O Thermoclostridium stercorarium catabolite repression with glucose and other readily metabolizable substrates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoclostridium stercorarium
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein xylanase A contains 19% carbohydrate, xylanase B contains 3% carbohydrate, xylanase C contains 4% carbohydrate Thermoclostridium stercorarium

Purification (Commentary)

Purification (Comment) Organism
xylanase A, B and C Thermoclostridium stercorarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
 Thermoclostridium stercorarium additional information
-
 ?
1,4-beta-D-xylan + H2O catabolite repression with glucose and other readily metabolizable substrates Thermoclostridium stercorarium ?
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O no hydrolysis Thermoclostridium stercorarium 4-nitrophenol + beta-D-xylopyranose
-
 ?
carboxymethylcellulose + H2O no activity Thermoclostridium stercorarium ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, xylanase A, SDS-PAGE Thermoclostridium stercorarium
monomer 1 * 44000, xylanase C, SDS-PAGE Thermoclostridium stercorarium
monomer 1* 72000, xylanase B, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Thermoclostridium stercorarium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 15 days, stable Thermoclostridium stercorarium
75
-
 1 h, xylanase A and C are stable, xylanase B retains 85% of its activity Thermoclostridium stercorarium
81
-
 half-life of xylanase A is 90 min, half-life of xylanase B is 2 min 30 s, half-life of xylanase C is 8 min Thermoclostridium stercorarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 at 65°C Thermoclostridium stercorarium

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 4°C, 24 h, xylanase B and C lose 50% of their activity Thermoclostridium stercorarium
4
-
 4°C, 24 h, complete inactivation of xylanase A Thermoclostridium stercorarium
5.5
-
 4°C, 24 h, xylanase A loses 50% of its activity Thermoclostridium stercorarium
7 12 4°C, 24 h, stable Thermoclostridium stercorarium