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Literature summary for 3.2.1.8 extracted from

  • Iefuji, H.; Chino, M.; Kato, M.; Iimura, Y.
    Acid xylanase from yeast Cryptococcus sp. S-2: purification, characterization, cloning, and sequencing (1996), Biosci. Biotechnol. Biochem., 60, 1331-1338.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Cryptococcus sp. (in: Fungi)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 x * 22000, SDS-PAGE Cryptococcus sp. (in: Fungi)

Organism

Organism UniProt Comment Textmining
Cryptococcus sp. (in: Fungi)
-
 S-2, acid xylanase
-

Purification (Commentary)

Purification (Comment) Organism
acid xylanase Cryptococcus sp. (in: Fungi)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87.5
-
-
 Cryptococcus sp. (in: Fungi)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
 Cryptococcus sp. (in: Fungi) additional information xylose + xylobiose + xylotriose + higher oligomers ?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Cryptococcus sp. (in: Fungi)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Cryptococcus sp. (in: Fungi)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
-
 Cryptococcus sp. (in: Fungi)

pH Range

pH Minimum pH Maximum Comment Organism
1 7 pH 1.0: about 75% of maximal activity, pH 7.0: about 40% of maximal activity Cryptococcus sp. (in: Fungi)

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 10 1 h, 30°C, 19% loss of activity at pH 1, 90% loss of activity at pH 10.0 Cryptococcus sp. (in: Fungi)