Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain KMAN-3 | Klebsiella oxytoca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | - |
- |
- |
Klebsiella oxytoca KUB-CW2-3 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Klebsiella oxytoca |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose resin column chromatography | Klebsiella oxytoca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
240 | - |
crude lysate, at pH 4.0 and 40°C | Klebsiella oxytoca |
11900 | - |
after 51fold purification, at pH 4.0 and 40°C | Klebsiella oxytoca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
defatted copra meal + H2O | - |
Klebsiella oxytoca | ? | - |
? | |
defatted copra meal + H2O | - |
Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
ivory nut mannan + H2O | lowest activity | Klebsiella oxytoca | ? | - |
? | |
ivory nut mannan + H2O | lowest activity | Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
konjac glucomannan + H2O | highest activity | Klebsiella oxytoca | ? | - |
? | |
konjac glucomannan + H2O | highest activity | Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
locust bean gum + H2O | - |
Klebsiella oxytoca | ? | - |
? | |
locust bean gum + H2O | - |
Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
additional information | no activity with carboxymethyl cellulose | Klebsiella oxytoca | ? | - |
? | |
additional information | no activity with carboxymethyl cellulose | Klebsiella oxytoca KUB-CW2-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Klebsiella oxytoca |
Synonyms | Comment | Organism |
---|---|---|
Beta-mannanase | - |
Klebsiella oxytoca |
endo-1,4-beta-mannanase | - |
Klebsiella oxytoca |
KMAN-3 | recombinant enzyme | Klebsiella oxytoca |