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Literature summary for 3.2.1.78 extracted from
- Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline beta-mannanase from an alkaliphilic Bacillus clausii strain S10 (2018), Microb. Cell Fact., 17, 124 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 144% activity at 1 mM | Alkalihalobacillus clausii |  |
| SDS | 106.2% activity at 1% (w/v) | Alkalihalobacillus clausii | |
| Urea | 122.7% activity at 5 mM | Alkalihalobacillus clausii | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells and Bacillus subtilis WB600 cells | Alkalihalobacillus clausii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Alkalihalobacillus clausii | |
| Co2+ | 32% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Cu2+ | 75.1% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Fe2+ | 1.5% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Fe3+ | complete inhibition at 1 mM | Alkalihalobacillus clausii | |
| guanidine hydrochloride | 20.5% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Hg2+ | complete inhibition at 1 mM | Alkalihalobacillus clausii | |
| Mn2+ | 44.8% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Ni2+ | 22.1% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Pb2+ | 1.0% residual activity at 1 mM | Alkalihalobacillus clausii | |
| Zn2+ | 1.1% residual activity at 1 mM | Alkalihalobacillus clausii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 112.3% activity at 1 mM | Alkalihalobacillus clausii | |
| Mg2+ | 108.4% activity at 1 mM | Alkalihalobacillus clausii | |
| Rb+ | 105.7% activity at 1 mM | Alkalihalobacillus clausii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalobacillus clausii | - |
- |
- |
| Alkalihalobacillus clausii S10 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Alkalihalobacillus clausii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Alkalihalobacillus clausii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | 18.1% activity compared to konjac glucomannan | Alkalihalobacillus clausii | ? | - |
? | |
| guar gum + H2O | 18.1% activity compared to konjac glucomannan | Alkalihalobacillus clausii S10 | ? | - |
? | |
| konjac glucomannan + H2O | 100% activity | Alkalihalobacillus clausii | ? | - |
? | |
| konjac glucomannan + H2O | 100% activity | Alkalihalobacillus clausii S10 | ? | - |
? | |
| locust bean gum + H2O | 88.2% activity compared to konjac glucomannan | Alkalihalobacillus clausii | ? | - |
? | |
| locust bean gum + H2O | 88.2% activity compared to konjac glucomannan | Alkalihalobacillus clausii S10 | ? | - |
? | |
| mannotetraose + H2O | - |
Alkalihalobacillus clausii | ? | - |
? | |
| mannotetraose + H2O | - |
Alkalihalobacillus clausii S10 | ? | - |
? | |
| additional information | the enzyme shows no activity towards carboxymethyl cellulose, xylan, glucan, pectin, chitin, soluble starch, or alpha-1,6-linked yeast mannan. Also, the enzyme shows no activity on 4-nitrophenyl beta- and alpha-mannosides, beta- and alpha-galactosides, and beta- and alpha-glucosides | Alkalihalobacillus clausii | ? | - |
? | |
| additional information | the enzyme shows no activity towards carboxymethyl cellulose, xylan, glucan, pectin, chitin, soluble starch, or alpha-1,6-linked yeast mannan. Also, the enzyme shows no activity on 4-nitrophenyl beta- and alpha-mannosides, beta- and alpha-galactosides, and beta- and alpha-glucosides | Alkalihalobacillus clausii S10 | ? | - |
? | |
| sesbania gum + H2O | 26% activity compared to konjac glucomannan | Alkalihalobacillus clausii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Alkalihalobacillus clausii |
| ? | x * 34300, calculated from amino acid sequence | Alkalihalobacillus clausii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Beta-mannanase | - |
Alkalihalobacillus clausii |
| ManA | - |
Alkalihalobacillus clausii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Alkalihalobacillus clausii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the enzyme exhibits god thermal stability at temperatures up to 70°C | Alkalihalobacillus clausii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 109.9 | - |
guar gum | at pH 9.5 and 75°C | Alkalihalobacillus clausii | |
| 164.7 | - |
sesbania gum | at pH 9.5 and 75°C | Alkalihalobacillus clausii | |
| 1238.9 | - |
konjac glucomannan | at pH 9.5 and 75°C | Alkalihalobacillus clausii | |
| 1376.1 | - |
locust bean gum | at pH 9.5 and 75°C | Alkalihalobacillus clausii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Alkalihalobacillus clausii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | more than 50% activity is retained at pH range 5.0-10.0, while there are 32.5% and 34.1% of relative activity at pH 6.0 and 11.0, respectively | Alkalihalobacillus clausii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11.5 | the enzyme is stable over an alkaline pH range from 6.0 to 11.5, in which more than 55% of the original enzyme activity is retained after 6 h at 30°C. The residual activity declines when the pH values are lower than 5.5 or higher than 11.5, and 20.5% and 25.8% of original activity is retained after pretreatment at pH 5.0 and 12.0, respectively | Alkalihalobacillus clausii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Alkalihalobacillus clausii | calculated from amino acid sequence | - |
4.6 |
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