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Literature summary for 3.2.1.78 extracted from
- A thermotolerant endo-1,4-beta-mannanase from Trichoderma virens UKM1 Cloning, recombinant expression and characterization (2016), J. Mol. Catal. B, 125, 49-57 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris X33 cells | Trichoderma virens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma virens | A0A0N9LQI1 | - |
- |
| Trichoderma virens UKM1 | A0A0N9LQI1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Trichoderma virens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| konjac glucomannan + H2O | - |
Trichoderma virens | ? | - |
? |  |
| konjac glucomannan + H2O | - |
Trichoderma virens UKM1 | ? | - |
? | |
| locust bean gum + H2O | - |
Trichoderma virens | ? | - |
? | |
| locust bean gum + H2O | - |
Trichoderma virens UKM1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000-70000, His6-tagged enzyme, SDS-PAGE | Trichoderma virens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-beta-mannanase | - |
Trichoderma virens |
| Man | - |
Trichoderma virens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Trichoderma virens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | the enzyme shows 70% of its maximum activity at 80°C | Trichoderma virens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | the enzyme shows good stability along 20-65°C and retains more than 60% of its activity for up to 1 h at 60°C. The enzyme has a half-life of 26xa0min at 70°C | Trichoderma virens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Trichoderma virens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | the enzyme has a wide pH stability range of pH 3.0-9.0 | Trichoderma virens |
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