Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus(DE3) cells | Streptomyces sp. NRRL B-24484 |
General Stability | Organism |
---|---|
although deleting carbohydrate binding module 10 increases the catalytic efficiency, its disruption decreases the pH, solvent and detergent stability of the enzyme | Streptomyces sp. NRRL B-24484 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Triton X-100 and Tween-20 (10% (v/v) | Streptomyces sp. NRRL B-24484 | |
SDS | the enzyme shows about 45% activity in the presence of 10% (w/v) SDS | Streptomyces sp. NRRL B-24484 |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | the enzyme retains 100% activity in the presence of 20% (v/v) acetone | Streptomyces sp. NRRL B-24484 |
Ethanol | the enzyme retains about 85% activity in the presence of 20% (v/v) ethanol | Streptomyces sp. NRRL B-24484 |
isopropanol | the enzyme retains about 65% activity in the presence of 20% (v/v) isopropanol | Streptomyces sp. NRRL B-24484 |
Methanol | the enzyme retains about 80% activity in the presence of 20% (v/v) methanol | Streptomyces sp. NRRL B-24484 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. NRRL B-24484 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Streptomyces sp. NRRL B-24484 |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid agarose column chromatography and HiTrap Q column chromatography | Streptomyces sp. NRRL B-24484 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactomannan + H2O | - |
Streptomyces sp. NRRL B-24484 | ? | - |
? | |
glucomannan + H2O | highest activity | Streptomyces sp. NRRL B-24484 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GH 134 beta-1,4-mannanase | - |
Streptomyces sp. NRRL B-24484 |
GH134 | - |
Streptomyces sp. NRRL B-24484 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces sp. NRRL B-24484 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the enzyme shows 30% and 60% of the maximal activity after an incubation at 100°C for 120 min and 80°C for 60 min, respectively | Streptomyces sp. NRRL B-24484 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
galactomannan | at pH 5.0 and 40°C | Streptomyces sp. NRRL B-24484 | |
340 | - |
glucomannan | at pH 5.0 and 40°C | Streptomyces sp. NRRL B-24484 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Streptomyces sp. NRRL B-24484 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | maximally active between pH 4.0 and 6.5 | Streptomyces sp. NRRL B-24484 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme retains more than 80% of the maximum activity after 90 min at 30°C within a pH range of 3.0-10.0 | Streptomyces sp. NRRL B-24484 |