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Literature summary for 3.2.1.78 extracted from

  • Nadaroglu, H.; Sonmez, Z.
    Purification of an endo-beta 1,4-mannanase from Clitocybe geotropa and immobilization on chitosan-coated magnetite nanoparticles Application for fruit juices (2016), Dig. J. Nanomater. Biostruct., 11, 685-697 .
No PubMed abstract available

Application

Application Comment Organism
food industry the enzyme is used for clarification of fruit juices such as grape, peach, orange and pomegranate juices Infundibulicybe geotropa

General Stability

General Stability Organism
the activity of enzyme immobilized onto chitosan coated magnetic nanoparticles is lost 21.4% after 10 cycles of reuse Infundibulicybe geotropa

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 89.7% residual activity at 5 mM Infundibulicybe geotropa
Zn2+ 90.9% residual activity at 5 mM Infundibulicybe geotropa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 133.4% activity at 5 mM Infundibulicybe geotropa
Cu2+ 187.9% activity at 1 mM Infundibulicybe geotropa
Fe2+ 118.2% activity at 5 mM Infundibulicybe geotropa
Hg2+ 454.6% activity at 5 mM Infundibulicybe geotropa
Mg2+ 109% activity at 1 mM Infundibulicybe geotropa

Organism

Organism UniProt Comment Textmining
Infundibulicybe geotropa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Infundibulicybe geotropa

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephacryl S-200 gel filtration Infundibulicybe geotropa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.475
-
crude extract, at pH 5.0 and 50°C Infundibulicybe geotropa
83.81
-
after 178.4fold purification, at pH 5.0 and 50°C Infundibulicybe geotropa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Infundibulicybe geotropa ?
-
?
cellulose + H2O
-
Infundibulicybe geotropa ?
-
?
locust bean gum + H2O highest activity Infundibulicybe geotropa mannose + ?
-
?
pectin + H2O
-
Infundibulicybe geotropa ?
-
?
sodium phytate + H2O
-
Infundibulicybe geotropa ?
-
?
starch + H2O
-
Infundibulicybe geotropa ?
-
?

Subunits

Subunits Comment Organism
? x * 39500, SDS-PAGE Infundibulicybe geotropa

Synonyms

Synonyms Comment Organism
endo-beta 1,4-mannanase
-
Infundibulicybe geotropa
mannan endo-1.4-beta-D-mannosidase
-
Infundibulicybe geotropa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
free enzyme Infundibulicybe geotropa
60
-
immobilized enzyme Infundibulicybe geotropa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Infundibulicybe geotropa

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 the immobilized enzyme exhibits more than 50% activity between pH 3.5 and 8.0 Infundibulicybe geotropa
4 6.5 the free enzyme exhibits more than 50% activity between pH 4.0 and 6.5 Infundibulicybe geotropa