Application | Comment | Organism |
---|---|---|
food industry | the enzyme is used for clarification of fruit juices such as grape, peach, orange and pomegranate juices | Infundibulicybe geotropa |
General Stability | Organism |
---|---|
the activity of enzyme immobilized onto chitosan coated magnetic nanoparticles is lost 21.4% after 10 cycles of reuse | Infundibulicybe geotropa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 89.7% residual activity at 5 mM | Infundibulicybe geotropa | |
Zn2+ | 90.9% residual activity at 5 mM | Infundibulicybe geotropa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 133.4% activity at 5 mM | Infundibulicybe geotropa | |
Cu2+ | 187.9% activity at 1 mM | Infundibulicybe geotropa | |
Fe2+ | 118.2% activity at 5 mM | Infundibulicybe geotropa | |
Hg2+ | 454.6% activity at 5 mM | Infundibulicybe geotropa | |
Mg2+ | 109% activity at 1 mM | Infundibulicybe geotropa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Infundibulicybe geotropa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Infundibulicybe geotropa |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephacryl S-200 gel filtration | Infundibulicybe geotropa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.475 | - |
crude extract, at pH 5.0 and 50°C | Infundibulicybe geotropa |
83.81 | - |
after 178.4fold purification, at pH 5.0 and 50°C | Infundibulicybe geotropa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Infundibulicybe geotropa | ? | - |
? | |
cellulose + H2O | - |
Infundibulicybe geotropa | ? | - |
? | |
locust bean gum + H2O | highest activity | Infundibulicybe geotropa | mannose + ? | - |
? | |
pectin + H2O | - |
Infundibulicybe geotropa | ? | - |
? | |
sodium phytate + H2O | - |
Infundibulicybe geotropa | ? | - |
? | |
starch + H2O | - |
Infundibulicybe geotropa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39500, SDS-PAGE | Infundibulicybe geotropa |
Synonyms | Comment | Organism |
---|---|---|
endo-beta 1,4-mannanase | - |
Infundibulicybe geotropa |
mannan endo-1.4-beta-D-mannosidase | - |
Infundibulicybe geotropa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free enzyme | Infundibulicybe geotropa |
60 | - |
immobilized enzyme | Infundibulicybe geotropa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Infundibulicybe geotropa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | the immobilized enzyme exhibits more than 50% activity between pH 3.5 and 8.0 | Infundibulicybe geotropa |
4 | 6.5 | the free enzyme exhibits more than 50% activity between pH 4.0 and 6.5 | Infundibulicybe geotropa |