Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Paenibacillus sp. HY-8 | |
Sodium azide | - |
Paenibacillus sp. HY-8 | |
Triton X-100 | - |
Paenibacillus sp. HY-8 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 28% inhibition at 5 mM | Paenibacillus sp. HY-8 | |
EDTA | 28% inhibition at 5 mM | Paenibacillus sp. HY-8 | |
Hg2+ | complete inhibition at 1 mM | Paenibacillus sp. HY-8 | |
N-bromosuccinimide | complete inhibition at 5 mM | Paenibacillus sp. HY-8 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Paenibacillus sp. HY-8 | |
Ca2+ | activates | Paenibacillus sp. HY-8 | |
Co2+ | 1.3fold increase of activity at 1 mM | Paenibacillus sp. HY-8 | |
Mn2+ | 1.3fold increase of activity at 1 mM | Paenibacillus sp. HY-8 | |
additional information | the stimulatory or inhibitory effect of divalent cations (each 1 mM) including Ni2+, Zn2+, and Sn2+ on the locust bean gum-degrading activity of the enzyme is negligible | Paenibacillus sp. HY-8 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. HY-8 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Paenibacillus sp. HY-8 |
Purification (Comment) | Organism |
---|---|
CM-Sepharose resin column chromatography and Superdex 200 gel filtration | Paenibacillus sp. HY-8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guar gum + H2O | - |
Paenibacillus sp. HY-8 | ? | - |
? | |
ivory nut mannan + H2O | - |
Paenibacillus sp. HY-8 | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose | - |
? | |
locust bean gum + H2O | - |
Paenibacillus sp. HY-8 | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose | - |
? | |
mannobiose + H2O | degradation of mannobiose yields a mixture of mannose (16.6 %) and mannobiose (83.4 %) | Paenibacillus sp. HY-8 | mannose + mannobiose | - |
? | |
mannopentaose + H2O | degradation of mannopentaose yields a mixture of mannose (3.1 %), mannobiose (11.2 %), mannotriose (41.1%), mannotetraose (21.7%), and mannopentaose (22.9%) | Paenibacillus sp. HY-8 | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose | - |
? | |
mannotetraose + H2O | degradation of mannotetraose yields a mixture of mannose (2.4 %), mannobiose (9.5 %) and mannotriose (62.3%) and mannotetraose (25.8%) | Paenibacillus sp. HY-8 | mannose + mannobiose + mannotriose + mannotetraose | - |
? | |
mannotriose + H2O | degradation of mannotriose yields a mixture of mannose (2.8 %), mannobiose (11.8 %) and mannotriose (85.4%) | Paenibacillus sp. HY-8 | mannose + mannobiose + mannotriose | - |
? | |
additional information | no activity with carboxymethyl cellulose, soluble starch, birchwood xylan, and pectin | Paenibacillus sp. HY-8 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Paenibacillus sp. HY-8 |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-mannanase | - |
Paenibacillus sp. HY-8 |
ManP | - |
Paenibacillus sp. HY-8 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Paenibacillus sp. HY-8 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | the enzyme shows 100%, 80%, 50%, 20% and no activity after 1 h at 37°C, 45°C, 50°C, 55°C, and 60°C, respectively | Paenibacillus sp. HY-8 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Paenibacillus sp. HY-8 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | the enzyme maintains over 85 % of its original biocatalytic activity toward locust bean gum after pre-incubation for 1 h from pH 4.5 to 9.5 | Paenibacillus sp. HY-8 |