Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.78 extracted from

  • Kim, D.Y.; Chung, C.W.; Cho, H.Y.; Rhee, Y.H.; Shin, D.H.; Son, K.H.; Park, H.Y.
    Biocatalytic characterization of an endo-beta-1,4-mannanase produced by Paenibacillus sp. strain HY-8 (2017), Biotechnol. Lett., 39, 149-155 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-ethylmaleimide
-
Paenibacillus sp. HY-8
Sodium azide
-
Paenibacillus sp. HY-8
Triton X-100
-
Paenibacillus sp. HY-8

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 28% inhibition at 5 mM Paenibacillus sp. HY-8
EDTA 28% inhibition at 5 mM Paenibacillus sp. HY-8
Hg2+ complete inhibition at 1 mM Paenibacillus sp. HY-8
N-bromosuccinimide complete inhibition at 5 mM Paenibacillus sp. HY-8

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Paenibacillus sp. HY-8
Ca2+ activates Paenibacillus sp. HY-8
Co2+ 1.3fold increase of activity at 1 mM Paenibacillus sp. HY-8
Mn2+ 1.3fold increase of activity at 1 mM Paenibacillus sp. HY-8
additional information the stimulatory or inhibitory effect of divalent cations (each 1 mM) including Ni2+, Zn2+, and Sn2+ on the locust bean gum-degrading activity of the enzyme is negligible Paenibacillus sp. HY-8

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. HY-8
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Paenibacillus sp. HY-8

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose resin column chromatography and Superdex 200 gel filtration Paenibacillus sp. HY-8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guar gum + H2O
-
Paenibacillus sp. HY-8 ?
-
?
ivory nut mannan + H2O
-
Paenibacillus sp. HY-8 mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose
-
?
locust bean gum + H2O
-
Paenibacillus sp. HY-8 mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose
-
?
mannobiose + H2O degradation of mannobiose yields a mixture of mannose (16.6 %) and mannobiose (83.4 %) Paenibacillus sp. HY-8 mannose + mannobiose
-
?
mannopentaose + H2O degradation of mannopentaose yields a mixture of mannose (3.1 %), mannobiose (11.2 %), mannotriose (41.1%), mannotetraose (21.7%), and mannopentaose (22.9%) Paenibacillus sp. HY-8 mannose + mannobiose + mannotriose + mannotetraose + mannopentaose
-
?
mannotetraose + H2O degradation of mannotetraose yields a mixture of mannose (2.4 %), mannobiose (9.5 %) and mannotriose (62.3%) and mannotetraose (25.8%) Paenibacillus sp. HY-8 mannose + mannobiose + mannotriose + mannotetraose
-
?
mannotriose + H2O degradation of mannotriose yields a mixture of mannose (2.8 %), mannobiose (11.8 %) and mannotriose (85.4%) Paenibacillus sp. HY-8 mannose + mannobiose + mannotriose
-
?
additional information no activity with carboxymethyl cellulose, soluble starch, birchwood xylan, and pectin Paenibacillus sp. HY-8 ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Paenibacillus sp. HY-8

Synonyms

Synonyms Comment Organism
endo-beta-1,4-mannanase
-
Paenibacillus sp. HY-8
ManP
-
Paenibacillus sp. HY-8

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Paenibacillus sp. HY-8

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 60 the enzyme shows 100%, 80%, 50%, 20% and no activity after 1 h at 37°C, 45°C, 50°C, 55°C, and 60°C, respectively Paenibacillus sp. HY-8

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Paenibacillus sp. HY-8

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9.5 the enzyme maintains over 85 % of its original biocatalytic activity toward locust bean gum after pre-incubation for 1 h from pH 4.5 to 9.5 Paenibacillus sp. HY-8