Cloned (Comment) | Organism |
---|---|
cloning in Escherichia coli strain JM109, functional overexpression in Pichia pastoris strain GS115, the enzyme is secreted | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | - |
Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
EDTA | - |
Bacillus subtilis | |
Fe2+ | - |
Bacillus subtilis | |
Fe3+ | - |
Bacillus subtilis | |
Hg2+ | - |
Bacillus subtilis | |
SDS | - |
Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Bacillus subtilis | |
additional information | Na+, Li+, NH4+, Ca2+, Mg2+ and Ba2+ have no effect on the recombinant mannanase activity | Bacillus subtilis | |
NaCl | activates 150% at 100 mg/ml | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | D0UH99 | isolated from soil sample of Heilongjiang Liangshui National Nature Reserve | - |
Bacillus subtilis WD23 | D0UH99 | isolated from soil sample of Heilongjiang Liangshui National Nature Reserve | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 9.7fold from Pichia pastoris strain GS115 culture supernatant to homogeneity by ammonium sulfate fractionation, dialysis, PEG 20000 fractionation, anion exchange chromatography, ultrafiltration, and gel filtration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4449 | - |
purified recombinant enzyme, pH 6.3, 70°C | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | recombinant enzyme, over 80% of maximal activity within this range | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
recombinant enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 9.1 | recombinant enzyme, more than 80% of the maximum activity at pH 4.8-7.8, the enzyme activity drops rapidly when at below pH 4.8 or above pH 9.1 | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.8 | purified recombinant enzyme, over 80% remaning after 1 h | Bacillus subtilis |
6.3 | 7.1 | purified recombinant enzyme, over 80% remaning after 2 h | Bacillus subtilis |