Cloned (Comment) | Organism |
---|---|
gene manA, recombinant expression in and secretion from Yarrowia lipolytica | Aspergillus aculeatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus aculeatus | the enzyme is involved in release of mannooligosaccharides from spent coffee ground cleaving the backbone at random locations in galactomannan, glucomannan, galactoglucomannan and mannan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus aculeatus | - |
gene manA | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme from Yarrowia lipolytica culture supernatant by ultrafiltration | Aspergillus aculeatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme in Yarrowia lipolytica is produced with an activity of 183.5 U/ml and 0.23 mg protein/ml | Aspergillus aculeatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
locust bean gum + H2O | - |
Aspergillus aculeatus | ? | - |
? | |
additional information | the enzyme is involved in release of mannooligosaccharides from spent coffee ground cleaving the backbone at random locations in galactomannan, glucomannan, galactoglucomannan and mannan | Aspergillus aculeatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4,D-mannanase | - |
Aspergillus aculeatus |
ManA | - |
Aspergillus aculeatus |
ManA/HmA | - |
Aspergillus aculeatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
recombinant enzyme | Aspergillus aculeatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | activity range, profile overview | Aspergillus aculeatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
glycerol at 30-40%, potassium sorbate/sodium benzoate at 0.2%, and sorbitol at 30-35% enhances enzyme thermostability and activity, overview | Aspergillus aculeatus |
60 | - |
a combination of mannanase with 0.2% sodium benzoate and 30% or 35% sorbitol presents the highest relative activity of about 150% when incubated at 60°C for 4 h, the control retains 63% of the initial activity | Aspergillus aculeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
assay at | Aspergillus aculeatus |
Organism | Comment | Expression |
---|---|---|
Aspergillus aculeatus | recombinant enzyme production is improved by 150% via supplementation with 0.2% sodium benzoate and 35% sorbitol as a preservative and stabiliser, respectively | up |