Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium azide | hydrolysis of locust bean gum is enhanced by approximately 1.45fold in poresence of sodium azide | Cellulosimicrobium sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cellulosimicrobium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | more than 50% inhibition | Cellulosimicrobium sp. | |
EDTA | 5 mM, 20% residaul activity | Cellulosimicrobium sp. | |
Fe2+ | more than 50% inhibition | Cellulosimicrobium sp. | |
Zn2+ | more than 50% inhibition | Cellulosimicrobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43767 | - |
x * 44000, SDS-PAGE, x * 43767, calculated | Cellulosimicrobium sp. |
44000 | - |
x * 44000, SDS-PAGE, x * 43767, calculated | Cellulosimicrobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulosimicrobium sp. | G3FBL1 | member of glycosyl hydrolase family 5 | - |
Cellulosimicrobium sp. HY-13 | G3FBL1 | member of glycosyl hydrolase family 5 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a N-terminal signal peptide of 24 amino acids | Cellulosimicrobium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8498 | - |
substrate locust bean gum, pH 6.0, 50°C | Cellulosimicrobium sp. |
14710 | - |
substrate ivory nut mannan, pH 6.0, 50°C | Cellulosimicrobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ivory nut mannan + H2O | - |
Cellulosimicrobium sp. | mannotriose + mannotetraose + mannopentaose + mannohexaose | main products, at 14%, 25%, 20%, and 13%, respectively | ? | |
ivory nut mannan + H2O | - |
Cellulosimicrobium sp. HY-13 | mannotriose + mannotetraose + mannopentaose + mannohexaose | main products, at 14%, 25%, 20%, and 13%, respectively | ? | |
locust bean gum + H2O | - |
Cellulosimicrobium sp. | mannopentaose | 44%, main product | ? | |
locust bean gum + H2O | - |
Cellulosimicrobium sp. HY-13 | mannopentaose | 44%, main product | ? | |
additional information | no substrate: mannobiose, or 4-nitrophenyl sugar derivatives | Cellulosimicrobium sp. | ? | - |
? | |
additional information | no substrate: mannobiose, or 4-nitrophenyl sugar derivatives | Cellulosimicrobium sp. HY-13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE, x * 43767, calculated | Cellulosimicrobium sp. |
Synonyms | Comment | Organism |
---|---|---|
ManH | - |
Cellulosimicrobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cellulosimicrobium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 15 min | Cellulosimicrobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Cellulosimicrobium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 1 h, more than 80% residual activity | Cellulosimicrobium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cellulosimicrobium sp. | calculated | - |
4.6 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme strongly binds to ivory nut mannan, Avicel, chitosan, and chitin, but does not attach to curdlan, insoluble oat spelt xylan, lignin, or poly(3-hydroxybutyrate) | Cellulosimicrobium sp. |