Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
S289W | mutation lowers KM for mannooligosaccharides by 30-45% and increases transglycosylation yield by 50% compared to wild-type | Aspergillus nidulans |
W283S | mutation in subsite +1, mutation results in increase in KM value, reduction in the transglycosylation yield by 30-45% and decrease in activity towards mannans | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
1 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
1.3 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
1.6 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
1.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
1.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
2.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
2.9 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43847 | - |
x * 56000, SDS-PAGE, x * 43847, calculated | Aspergillus nidulans |
46644 | - |
x * 56000, SDS-PAGE, x * 46644, calculated | Aspergillus nidulans |
56000 | - |
x * 56000, SDS-PAGE, x * 43847, calculated | Aspergillus nidulans |
56000 | - |
x * 56000, SDS-PAGE, x * 46644, calculated | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q5AZ53 | member of glycosyl hydrolase family 5 | - |
Aspergillus nidulans | Q5B7X2 | member of glycosyl hydrolase family 5 | - |
Aspergillus nidulans FGSC A4 | Q5AZ53 | member of glycosyl hydrolase family 5 | - |
Aspergillus nidulans FGSC A4 | Q5B7X2 | member of glycosyl hydrolase family 5 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | treatment by endoglycosidase H decreases molecular mass to 46000 Da | Aspergillus nidulans |
glycoprotein | treatment by endoglycosidase H decreases molecular mass to 51000 Da | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guar gum glucomannan + H2O | - |
Aspergillus nidulans | ? | - |
? | |
guar gum glucomannan + H2O | - |
Aspergillus nidulans FGSC A4 | ? | - |
? | |
konjac glucomannan + H2O | - |
Aspergillus nidulans | ? | - |
? | |
konjac glucomannan + H2O | - |
Aspergillus nidulans FGSC A4 | ? | - |
? | |
locust bean gum glucomannan + H2O | - |
Aspergillus nidulans | ? | - |
? | |
locust bean gum glucomannan + H2O | - |
Aspergillus nidulans FGSC A4 | ? | - |
? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | 2 Man-beta-1,4-Man | 64% of product | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | 2 Man-beta-1,4-Man | 68% of product | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans FGSC A4 | 2 Man-beta-1,4-Man | 68% of product | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans FGSC A4 | 2 Man-beta-1,4-Man | 64% of product | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man | sole products | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man | sole products | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | 88% of products | ? | |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | 88% of products | ? | |
additional information | enzyme displays good transgycosylation activity using mannotriose, melezitose and isomaltotriose as acceptors | Aspergillus nidulans | ? | - |
? | |
additional information | enzyme displays transglycosylation activity using only mannotriose as acceptor | Aspergillus nidulans | ? | - |
? | |
additional information | enzyme displays good transgycosylation activity using mannotriose, melezitose and isomaltotriose as acceptors | Aspergillus nidulans FGSC A4 | ? | - |
? | |
additional information | enzyme displays transglycosylation activity using only mannotriose as acceptor | Aspergillus nidulans FGSC A4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE, x * 43847, calculated | Aspergillus nidulans |
? | x * 56000, SDS-PAGE, x * 46644, calculated | Aspergillus nidulans |
Synonyms | Comment | Organism |
---|---|---|
ManA | - |
Aspergillus nidulans |
ManC | - |
Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
3 h, 95% residual activity | Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
67 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
98 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
112 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
117 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
134 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
148 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
193 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Aspergillus nidulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | more than 95% of maximum activity | Aspergillus nidulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
27 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
36 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
61 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
109 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
115 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
118 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
215 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans |