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Literature summary for 3.2.1.78 extracted from

  • Songsiriritthigul, C.; Buranabanyat, B.; Haltrich, D.; Yamabhai, M.
    Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-beta-mannosidase from Bacillus licheniformis in Escherichia coli (2010), Microb. Cell Fact., 9, 20.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values are 14.9 mg/ml, 17.5 mg/ml, and 15.2 mg/ml for glucomannan, locust bean gum and beta-D-mannan, respectively Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 45000, SDS-PAGE, x * 41000, calculated Bacillus licheniformis
45000
-
 x * 45000, SDS-PAGE, x * 41000, calculated Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis D0UHB3 gene name ydhT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus licheniformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1672
-
 50°C, pH 6.0 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-D-mannan + H2O 166% of the activity with locust bean gum Bacillus licheniformis ? main products are manno-oligosaccharides of 2-6 residues and mannose ?
glucomannan + H2O 219% of the activity with locust bean gum Bacillus licheniformis ?
-
 ?
locust bean gum + H2O
-
 Bacillus licheniformis ? main products are manno-oligosaccharides of 2-6 residues and mannose ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE, x * 41000, calculated Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 after incubation for 30 min, stable at pH 5-12. After incubation for 24 h, stable at pH 6-9. Half-life about 80 h at pH 6.0 Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18200
-
 beta-1,4-D-mannan 50°C, pH 6.0 Bacillus licheniformis
21000
-
 glucomannan 50°C, pH 6.0 Bacillus licheniformis
31200
-
 locust bean gum 50°C, pH 6.0 Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 12 after incubation at 50°C for 30 min Bacillus licheniformis
6 9 after incubation at 50°C for 24 h Bacillus licheniformis