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Literature summary for 3.2.1.78 extracted from

  • Wang, A.; Li, J.; Zhang, B.; Xu, X.; Bewley, J.D.
    Expression and location of endo-beta-mannanase during the ripening of tomato fruit, and the relationship between its activity and softening (2009), J. Plant Physiol., 166, 1672-1684.
    View publication on PubMed

Application

Application Comment Organism
agriculture when endo-beta-mannanase activity is much reduced by RNAi and antisense RNA strategies, their firmness is higher compared to those of control fruits at the turning and orange-color stages, but at the red-ripe stage firmness is similar between the two fruit-types Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall transient expression in onion epidermal cells of endo-beta-mannanase trancripts fused to green fluorescent protein results in the expressed enzyme being localized to the cell walls. This occurs initially during ripening in the skin and outer pericarp of the fruit, and later in the skin and throughout the pericarp Solanum lycopersicum 5618
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Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q8L5J1
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Source Tissue

Source Tissue Comment Organism Textmining
fruit isoform Man4a is expressed in the fruit cell wall at all ripening stages, but it is not active during the initial green stage. This is not due to the presence of inhibitors of its activity, nor due to changes in its mRNA sequence Solanum lycopersicum
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Synonyms

Synonyms Comment Organism
Man4A
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Solanum lycopersicum

General Information

General Information Comment Organism
physiological function when endo-beta-mannanase activity is much reduced by RNAi and antisense RNA strategies, their firmness is higher compared to those of control fruits at the turning and orange-color stages, but at the red-ripe stage firmness is similar between the two fruit-types Solanum lycopersicum