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Literature summary for 3.2.1.78 extracted from

  • Blibech, M.; Ghorbel, R.E.; Fakhfakh, I.; Ntarima, P.; Piens, K.; Bacha, A.B.; Chaabouni, S.E.
    Purification and characterization of a low molecular weight of beta-mannanase from Penicillium occitanis Pol6 (2010), Appl. Biochem. Biotechnol., 160, 1227-1240.
    View publication on PubMed
Search for more literature for 3.2.1.78

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, 64% residual activity. Stimulating above 2.5 mM Penicillium sp. 'occitanis'
Co2+ 2.5 mM, 42% residual activity Penicillium sp. 'occitanis'
EDTA 2.5 mM, 77% residual activity Penicillium sp. 'occitanis'
Fe3+ 2.5 mM, 71% residual activity Penicillium sp. 'occitanis'
mannose
-
 Penicillium sp. 'occitanis'

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2.5 mM, 10% stimulation. Inhibition at 2.0 mM Penicillium sp. 'occitanis'
Cu2+ 2.5 mM, 9% stimulation Penicillium sp. 'occitanis'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 SDS-PAGE Penicillium sp. 'occitanis'

Organism

Organism UniProt Comment Textmining
Penicillium sp. 'occitanis'
-
 Pol6
-
Penicillium sp. 'occitanis' Pol6
-
 Pol6
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 5% carbohydrate content Penicillium sp. 'occitanis'

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium sp. 'occitanis'

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
129.3
-
 pH 4.0, 40°C Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
locust bean gum + H2O
-
 Penicillium sp. 'occitanis' ?
-
 ?
locust bean gum + H2O
-
 Penicillium sp. 'occitanis' Pol6 ?
-
 ?
additional information no substrate: carboxymethyl cellulose, oat spelt xylan, starch, pectin, 4-nitrophenyl-beta-D-mannopyranoside Penicillium sp. 'occitanis' ?
-
 ?
additional information no substrate: carboxymethyl cellulose, oat spelt xylan, starch, pectin, 4-nitrophenyl-beta-D-mannopyranoside Penicillium sp. 'occitanis' Pol6 ?
-
 ?

Synonyms

Synonyms Comment Organism
ManIII
-
 Penicillium sp. 'occitanis'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Penicillium sp. 'occitanis'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable for 30 min Penicillium sp. 'occitanis'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Penicillium sp. 'occitanis'

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 12
-
 Penicillium sp. 'occitanis'