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Literature summary for 3.2.1.78 extracted from

  • Jiang, Z.; Wei, Y.; Li, D.; Li, L.; Chai, P.; Kusakabe, I.
    High-level production, purification and characterization of a thermostable beta-mannanase from the newly isolated Bacillus subtilis WY34 (2006), Carbohydr. Polym., 66, 88-96.
No PubMed abstract available
Search for more literature for 3.2.1.78

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 54% residual activity Bacillus subtilis
EDTA 1 mM, 37% residual activity Bacillus subtilis
Mg2+ 1 mM, 53% residual activity Bacillus subtilis
Mn2+ 1 mM, 22% residual activity Bacillus subtilis
N-bromosuccinimide 1 mM, 3% residual activity Bacillus subtilis
Ni2+ 1 mM, 61% residual activity Bacillus subtilis
sodium dodecylsulfate 1 mM, 55% residual activity Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information apparent Km values of the mannanase for locust bean gum, guar gum and konjac powder are 7.6, 10.5 and 27.4 mg/ml, respectively Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 126% of initial activity Bacillus subtilis
Fe2+ 1 mM, 124% of initial activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39600
-
 x * 39600, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 induced by growth on konjac powder as carbon source at 50°C
-
Bacillus subtilis WY34
-
 induced by growth on konjac powder as carbon source at 50°C
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of 13.1% Bacillus subtilis

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8302
-
 pH 6.0, 50°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O poor substrate Bacillus subtilis ?
-
 ?
carboxymethyl cellulose + H2O poor substrate Bacillus subtilis WY34 ?
-
 ?
locust bean gum + H2O
-
 Bacillus subtilis ?
-
 ?
locust bean gum + H2O
-
 Bacillus subtilis WY34 ?
-
 ?
mannan + H2O source of substrate: copra Bacillus subtilis mannotetraose + mannobiose + mannotriose
-
 ?
mannan + H2O source of substrate: copra Bacillus subtilis WY34 mannotetraose + mannobiose + mannotriose
-
 ?
starch + H2O poor substrate Bacillus subtilis ?
-
 ?
starch + H2O poor substrate Bacillus subtilis WY34 ?
-
 ?
xylan + H2O poor substrate Bacillus subtilis ?
-
 ?
xylan + H2O poor substrate Bacillus subtilis WY34 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39600, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 at pH 6.0, stable up to Bacillus subtilis
75
-
 30 min, 90% loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10.1
-
 Bacillus subtilis