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Literature summary for 3.2.1.78 extracted from
- Purification and characterization of two forms of endo-beta-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749) (2004), Biochim. Biophys. Acta, 1674, 239-250.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | pulp production | Aspergillus fumigatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.07 | - |
low viscosity locust bean galactomannan | isozyme MAN I | Aspergillus fumigatus | |
| 3.12 | - |
low viscosity locust bean galactomannan | isozyme MAN II | Aspergillus fumigatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
isozyme MAN I, SDS-PAGE | Aspergillus fumigatus |
| 63000 | - |
isozyme MAN II, SDS-PAGE | Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
strain IMI 385708, formerly Thermomyces lanuginosus strain IMI 158749 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fast flow column chromatography | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Aspergillus fumigatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1638 A550/min/mg optical path 1cm,isozyme MAN I, OBR-LBG | Aspergillus fumigatus |
| additional information | - |
1763 A550/min/mg optical path 1cm, isozyme MAN II, OBR-LBG | Aspergillus fumigatus |
| additional information | - |
no activity against 4-nitrophenyl-alpha and -beta-mannopyranosides | Aspergillus fumigatus |
| 4.6 | - |
crude medium | Aspergillus fumigatus |
| 45.7 | - |
isozyme MAN I, after 9.9fold purification with DEAE Sepharose chromatography | Aspergillus fumigatus |
| 56.7 | - |
isozyme MAN II, after 12.3fold purification with DEAE Sepharose chromatography | Aspergillus fumigatus |
| 190 | - |
isozyme MAN I, mannan A | Aspergillus fumigatus |
| 215 | - |
isozyme MAN II, mannan A | Aspergillus fumigatus |
| 225.4 | - |
isozyme MAN II, after 49fold purification with Phenyl Sepharose chromatography | Aspergillus fumigatus |
| 294 | - |
isozyme MAN I, guar gum | Aspergillus fumigatus |
| 317 | - |
isozyme MAN II, guar gum | Aspergillus fumigatus |
| 471 | - |
isozyme MAN I, after 102.4fold purification with Phenyl Sepharose chromatography | Aspergillus fumigatus |
| 562 | - |
isozyme MAN I, locust bean gum | Aspergillus fumigatus |
| 627 | - |
isozyme MAN II, locust bean gum | Aspergillus fumigatus |
| 663 | - |
isozyme MAN I, low viscosity locust bean galactomannan | Aspergillus fumigatus |
| 751 | - |
isozyme MAN II, low viscosity locust bean galactomannan | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | - |
Aspergillus fumigatus | ? | - |
? |  |
| locust bean gum + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
| low viscosity locust bean galactomannan + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
| mannan A + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-mannanase | - |
Aspergillus fumigatus |
| MAN I | isozyme, major form | Aspergillus fumigatus |
| Man II | isozyme, minor form | Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
at pH 4.5 | Aspergillus fumigatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable up to 55°C | Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1935 | - |
low viscosity locust bean galactomannan | isozyme MAN I | Aspergillus fumigatus | |
| 2246 | - |
low viscosity locust bean galactomannan | isozyme MAN II | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
at 60°C | Aspergillus fumigatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Aspergillus fumigatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | - |
Aspergillus fumigatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus fumigatus | isozyme MAN II | 4.9 | 4.75 |
| Aspergillus fumigatus | isozyme MAN I | 5.2 | 4.9 |
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