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Literature summary for 3.2.1.78 extracted from

  • Le Nours, J.; Anderson, L.; Stoll, D.; Stalbrand, H.; Lo Leggio, L.
    The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi (2005), Biochemistry, 44, 12700-12708.
    View publication on PubMed
Search for more literature for 3.2.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Cellulomonas fimi

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion method at room temperature Cellulomonas fimi

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Cellulomonas fimi 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE, a stable fragment corresponding to about 460 residues and comprising the catalytic domain and 50 to 60 extra residues at the C-terminus Cellulomonas fimi

Organism

Organism UniProt Comment Textmining
Cellulomonas fimi
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.69
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man} Cellulomonas fimi
1.13
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man Cellulomonas fimi
1.95
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man Cellulomonas fimi
3.1
-
 Manbeta(1-4)Manbeta(1-4)Man Cellulomonas fimi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi mannose + Manbeta(1-4)Man
-
 ?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
-
 ?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi mannose + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man
-
 ?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O
-
 Cellulomonas fimi D-mannitol + Manbeta(1-4)Man-ol + D-mannose + Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man
-
 ?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-1,4-mannanase
-
 Cellulomonas fimi
Man26A-50K
-
 Cellulomonas fimi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Cellulomonas fimi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
0.41
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
1.17
-
 Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
11.64
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
52.42
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
77
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Cellulomonas fimi