Cloned (Comment) | Organism |
---|---|
expression of IDUA with and without a C-terminal endoplasmic reticulum-retention sequence SEKDEL, in seeds of Brassica napus and Nicotiana tabacum under the control of regulatory 5'-, signal-peptide-encoding-, and 3'-sequences from the arcelin 5-I gene of Phaseolus vulgaris | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76600 | - |
x * 76600, recombinant glycosylated enzyme expressed from Brassica napus seeds, SDS-PAGE, x * 76700, recombinant glycosylated enzyme expressed from Nicotiana tabacum seeds, SDS-PAGE | Homo sapiens |
76700 | - |
x * 76600, recombinant glycosylated enzyme expressed from Brassica napus seeds, SDS-PAGE, x * 76700, recombinant glycosylated enzyme expressed from Nicotiana tabacum seeds, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene IDUA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is N-glycosylated. The carboxy-terminal endoplasmic reticulum-retention motif, SEKDEL, influences the N-linked glycosylation of the recombinant enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme with and without a C-terminal ER-retention signal from seeds of Brassica napus and Nicotiana tabacum by lectinb affinity, immunoaffinity, and adsorption chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
purified recombinant enzyme expressed from Brassica napus seeds | Homo sapiens |
39.7 | - |
purified recombinant enzyme expressed from Nicotiana tabacum seeds | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-iduronoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-iduronic acid | - |
? | |
additional information | substrate specificity of recombinant enzyme with and without a C-terminal ER-retention signal from seeds of Brassica napus and Nicotiana tabacum, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76600, recombinant glycosylated enzyme expressed from Brassica napus seeds, SDS-PAGE, x * 76700, recombinant glycosylated enzyme expressed from Nicotiana tabacum seeds, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-iduronidase | - |
Homo sapiens |
IDUA | - |
Homo sapiens |