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Literature summary for 3.2.1.76 extracted from

  • Fu, L.; Miao, Y.; Lo, S.; Seto, T.; Sun, S.; Xu, Z.; Clemens, S.; Clarke, L.; Kermode, A.; Jiang, L.
    Production and characterization of soluble human lysosomal enzyme ?-iduronidase with high activity from culture media of transgenic tobacco BY-2 cells (2009), Plant Sci., 177, 668-675.
    View publication on PubMed
Search for more literature for 3.2.1.76

Application

Application Comment Organism
medicine hIDUA is used for enzyme replacement therapy in MPS I patients Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
IDUA cloning in Escherichia coli strain BL21, and overexpression in transgenic Nicotiana tabacum BY-2 cells, establishing and evaluation of the expression system, overview. A plant signal peptide is essential for proper expression and secretion of the glycosylated recombinant hIDUA into the cultured media of transgenic BY-2 cells, transfection by Agrobacterium tumefaciens, strain LBA4404 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 x * 78000, recombinant glycosylated enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Subunits

Subunits Comment Organism
? x * 78000, recombinant glycosylated enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha-iduronidase
-
 Homo sapiens
IDUA
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction enzyme deficiency causes mucopolysaccharidosis I, MCS I Homo sapiens