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Literature summary for 3.2.1.75 extracted from
- Purification and properties of a basic endo-beta-1,6-glucanase (BGN16.1) from the antagonistic fungus Trichoderma harzianum (1999), Eur. J. Biochem., 265, 245-151.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pustulan 0.8 mg/ml | Trichoderma harzianum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma harzianum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | 25000 | gel filtration | Trichoderma harzianum |
| 51000 | - |
x * 51000, SDS-PAGE | Trichoderma harzianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
CECT 2313 | - |
| Trichoderma harzianum CECT 2313 | - |
CECT 2313 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trichoderma harzianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | - |
- |
Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | Saccharomyces cerevisiae glucan | Trichoderma harzianum | glucose + laminari-oligosaccharides + gentio-oligosaccharides | - |
? |  |
| beta-glucan + H2O | Saccharomyces cerevisiae glucan | Trichoderma harzianum CECT 2313 | glucose + laminari-oligosaccharides + gentio-oligosaccharides | - |
? | |
| laminarin + H2O | - |
Trichoderma harzianum | gentiobiose + D-glucose | - |
? | |
| laminarin + H2O | - |
Trichoderma harzianum CECT 2313 | gentiobiose + D-glucose | - |
? | |
| pustulan + H2O | - |
Trichoderma harzianum | glucose + gentiobiose | glucose, gentiotetraose, gentiobiose | ? | |
| pustulan + H2O | - |
Trichoderma harzianum CECT 2313 | glucose + gentiobiose | glucose, gentiotetraose, gentiobiose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, SDS-PAGE | Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trichoderma harzianum |
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Release 2023.1 (January 2023)
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