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Literature summary for 3.2.1.75 extracted from
- Purification and properties of a beta-1,6-glucanase from Penicillium brefeldianum (1984), Biochem. J., 223, 707-714.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pustulan 2.78 mg/ml | Penicillium brefeldianum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium brefeldianum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Penicillium brefeldianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium brefeldianum | - |
QM 1873 | - |
| Penicillium brefeldianum QM 1873 | - |
QM 1873 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7080fold | Penicillium brefeldianum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Penicillium brefeldianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 405 | - |
- |
Penicillium brefeldianum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH6, 2 months, no loss of activity | Penicillium brefeldianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | Ecklonia radiata glucan, poor substrate | Penicillium brefeldianum | glucose + laminari-oligosaccharides + gentio-oligosaccharides | - |
? |  |
| beta-glucan + H2O | Ecklonia radiata glucan, poor substrate | Penicillium brefeldianum QM 1873 | glucose + laminari-oligosaccharides + gentio-oligosaccharides | - |
? | |
| carboxymethyl-pachyman + H2O | poor substrate | Penicillium brefeldianum | ? | - |
? | |
| carboxymethyl-pachyman + H2O | poor substrate | Penicillium brefeldianum QM 1873 | ? | - |
? | |
| gentiobiose + H2O | poor substrate | Penicillium brefeldianum | D-glucose | - |
? | |
| gentiobiose + H2O | poor substrate | Penicillium brefeldianum QM 1873 | D-glucose | - |
? | |
| pachyman + H2O | poor substrate | Penicillium brefeldianum | gentiobiose | - |
? | |
| pachyman + H2O | poor substrate | Penicillium brefeldianum QM 1873 | gentiobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Penicillium brefeldianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium brefeldianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
- |
Penicillium brefeldianum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | at -20°C | Penicillium brefeldianum |
| 5 | 8 | - |
Penicillium brefeldianum |
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Release 2023.1 (January 2023)
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