Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130300 | - |
gel filtration | Gecarcoidea natalis |
169300 | - |
calculated from amino acid sequence | Gecarcoidea natalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gecarcoidea natalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Macro-Prep DEAE column chromatography, methyl hydrophobic-interaction column chromatography, and Superdex 200 gel filtration | Gecarcoidea natalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut gland | - |
Gecarcoidea natalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.067 | - |
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication | Gecarcoidea natalis |
0.228 | - |
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication | Gecarcoidea natalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | glucosyl transfer reaction | Gecarcoidea natalis | D-glucose + cellotetraose + cellotriose | - |
? | |
cellotetraose + H2O | highest substrate preference for cellotriose and cellotetraose | Gecarcoidea natalis | D-glucose + cellotriose + cellobiose | - |
? | |
cellotriose + H2O | highest substrate preference for cellotriose and cellotetraose | Gecarcoidea natalis | D-glucose + cellobiose | - |
? | |
cellulose + H2O | the glucohydrolase attaches directly to crystalline cellulose to release D-glucose from free chain ends | Gecarcoidea natalis | D-glucose + ? | - |
? | |
additional information | no activity is detected towards carboxymethyl cellulose | Gecarcoidea natalis | ? | - |
? | |
additional information | the enzyme is able to synthesise some cellotetraose by the transglycosylation of smaller substrates | Gecarcoidea natalis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,4-beta-D-glucan glucanohydrolase | - |
Gecarcoidea natalis |
glucohydrolase | - |
Gecarcoidea natalis |