Literature summary for 3.2.1.74 extracted from

Jagtap, S.; Rao, M.
Fluorescence study on interactions of alpha-crystallin with the molten globule state of 1, 4-beta-D-glucan glucanohydrolase from Thermomonospora sp. induced by guanidine hydrochloride (2009), J. Fluoresc., 19, 967-973.

Organism

Organism	UniProt	Comment	Textmining
Thermomonospora sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
TSC by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration	Thermomonospora sp.

Renatured (Commentary)

Renatured (Comment)	Organism
denaturation studies using GdnCl indicate that TSC folds through a partially folded state that resembles molten globule at 1.8 M GdnCl. alpha-Crystallin chaperone-mediated in vitro folding, molecular mechanism, overview. Reconstitution of the active TSC is observed in 50 mM sodium phosphate buffer, pH 7.0, on cooling the alpha-crystallin-TSC-m complex to 4°C. Addition of alpha-crystallin to the molten globule-like intermediate of TSC complex initiates the refolding of TSC with 69% recovery of the biological activity of the enzyme	Thermomonospora sp.

Renatured (Comment)

Organism

denaturation studies using GdnCl indicate that TSC folds through a partially folded state that resembles molten globule at 1.8 M GdnCl. alpha-Crystallin chaperone-mediated in vitro folding, molecular mechanism, overview. Reconstitution of the active TSC is observed in 50 mM sodium phosphate buffer, pH 7.0, on cooling the alpha-crystallin-TSC-m complex to 4°C. Addition of alpha-crystallin to the molten globule-like intermediate of TSC complex initiates the refolding of TSC with 69% recovery of the biological activity of the enzyme

Thermomonospora sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxymethylcellulose + H2O	-	Thermomonospora sp.	?	-	?
additional information	fluorescence study on interactions of chaperone alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride	Thermomonospora sp.	?	-	?

Subunits

Subunits	Comment	Organism
More	fluorescence study on interactions of alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride	Thermomonospora sp.

Synonyms

Synonyms	Comment	Organism
1,4-beta-D-glucan glucanohydrolase	-	Thermomonospora sp.
Carboxymethyl cellulase	-	Thermomonospora sp.
TSC	-	Thermomonospora sp.