Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | calcium ions are not required for its function | Fibrobacter succinogenes subsp. succinogenes S85 |
Cloned (Comment) | Organism |
---|---|
into vector pET30a and expressed in Escherichia coli BL21(DE3) | Fibrobacter succinogenes subsp. succinogenes S85 |
Protein Variants | Comment | Organism |
---|---|---|
D166A | no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation | Fibrobacter succinogenes subsp. succinogenes S85 |
DELTAS168 | no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation | Fibrobacter succinogenes subsp. succinogenes S85 |
E612A | no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation | Fibrobacter succinogenes subsp. succinogenes S85 |
S168A | retains about 40% of activity compared to the wild-type | Fibrobacter succinogenes subsp. succinogenes S85 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Cel9D has 27% lower activity on medium-viscosity carboxymethyl cellulose compared to the activity on low-viscosity carboxymethyl cellulose. Is not inhibited by 10 mM EDTA | Fibrobacter succinogenes subsp. succinogenes S85 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
cellohexaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
0.9 | - |
cellopentaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
1.21 | - |
cellotetraose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
1.5 | 2 | cellotriose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
28 | - |
cellobiose | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Fibrobacter succinogenes subsp. succinogenes S85 | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
1 * 76000, SDS-PAGE | Fibrobacter succinogenes subsp. succinogenes S85 |
77000 | - |
sequence analysis | Fibrobacter succinogenes subsp. succinogenes S85 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fibrobacter succinogenes subsp. succinogenes S85 | B0FWI3 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants purified by immobilized metal affinity chromatography. Wild-type further purified by gel filtration | Fibrobacter succinogenes subsp. succinogenes S85 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.038 | - |
with avicel | Fibrobacter succinogenes subsp. succinogenes S85 |
0.25 | - |
with amorphous cellulose | Fibrobacter succinogenes subsp. succinogenes S85 |
0.275 | - |
with carboxymethyl cellulose | Fibrobacter succinogenes subsp. succinogenes S85 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acid swollen cellulose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | D-glucose + ? | - |
? | |
amorphous cellulose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
avicel + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
cellobiose + H2O | is degraded very slowly | Fibrobacter succinogenes subsp. succinogenes S85 | 2 D-glucose | - |
? | |
cellohexaose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | cellopentaose + D-glucose | - |
? | |
cellopentaose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
cellotetraose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
cellotriose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
additional information | no activity on laminarin, sophorose, laminaribiose, gentiobiose, p-nitrophenol-alpha-L-arabinofuranoside, p-nitrophenol-alpha-D-glucoside, p-nitrophenol-beta-D-cellobioside, p-nitrophenol-beta-D-fucoside, p-nitrophenol-beta-D-galactoside, p-nitrophenol-N-acetyl-beta-D-glucosaminide, p-nitrophenol-beta-D-glucoside, p-nitrophenol-beta-D-glucuronoside, p-nitrophenol-beta-D-lactoside, or p-nitrophenol-beta-D-maltoside | Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
p-nitrophenol beta-D-cellobioside + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | p-nitrophenol beta-D-glucopyranoside + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 76000, SDS-PAGE | Fibrobacter succinogenes subsp. succinogenes S85 |
Synonyms | Comment | Organism |
---|---|---|
1,4-beta-D-glucan glucohydrolase | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
Cel9D | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
family 9 glycoside hydrolase | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
GH9 | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Fibrobacter succinogenes subsp. succinogenes S85 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
cellobiose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
6.21 | - |
cellotriose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
16.3 | - |
cellohexaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
16.4 | - |
cellopentaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
18.6 | - |
cellotetraose | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Fibrobacter succinogenes subsp. succinogenes S85 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | retains 80% activity | Fibrobacter succinogenes subsp. succinogenes S85 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fibrobacter succinogenes subsp. succinogenes S85 | - |
- |
5.27 |