Literature summary for 3.2.1.74 extracted from

Bravo, V.; Paez, M.P.; Aoulad, M.; Reyes, A.
The influence of temperature upon the hydrolysis of cellobiose by beta-1,4-glucosidases from Aspergillus niger (2000), Enzyme Microb. Technol., 26, 614-620.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetic	Aspergillus niger
0.461	-	cellobiose	at 40ºC, pH 4.9	Aspergillus niger
1.26	-	cellobiose	at 50ºC, pH 4.9	Aspergillus niger
2.89	-	cellobiose	at 60ºC, pH 4.9	Aspergillus niger
3.12	-	cellobiose	at 70ºC, pH 4.9	Aspergillus niger
6.82	-	cellobiose	value extrapolated to 70ºC	Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellobiose + H2O	Aspergillus niger	hydrolysis of cellulose	2 D-glucose	-	ir

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no modification	-	Aspergillus niger

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellobiose + H2O	hydrolysis of cellulose	Aspergillus niger	2 D-glucose	-	ir
D(+)-cellobiose + H2O	-	Aspergillus niger	D-glucose	-	r

Synonyms

Synonyms	Comment	Organism
beta-1,4-glucosidase	-	Aspergillus niger
cellobiase	-	Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	60	loses none of its activity for a period of up to 5 h	Aspergillus niger
70	-	undergoes considerable deactivation within quite a short time	Aspergillus niger

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Release 2023.1 (January 2023)