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Literature summary for 3.2.1.74 extracted from
- Cellobiosidase from Ruminococcus albus (1988), Methods Enzymol., 160, 391-398.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Ruminococcus albus |  |
| iodoacetoamide | - |
Ruminococcus albus | |
| p-chloromercuribenzoate | - |
Ruminococcus albus | |
| Zn2+ | - |
Ruminococcus albus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Ruminococcus albus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
alpha,beta, 2 * 100000, SDS-PAGE | Ruminococcus albus |
| 200000 | - |
gel filtration | Ruminococcus albus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminococcus albus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 39fold | Ruminococcus albus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Ruminococcus albus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
20.8 OD405/mg | Ruminococcus albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Avicel + H2O | - |
Ruminococcus albus | D-glucose + cellotetraose + cellotriose + cellobiose | - |
? | |
| carboxymethyl cellulose + H2O | - |
Ruminococcus albus | ? | - |
? | |
| cellobiose + H2O | - |
Ruminococcus albus | D-glucose + cellotetraose + cellotriose | - |
ir | |
| cellooligomers + H2O | - |
Ruminococcus albus | cellobiose + cellooligomers | - |
ir | |
| cellopentaose + H2O | - |
Ruminococcus albus | D-glucose + ? | - |
ir | |
| cellotetraose + H2O | - |
Ruminococcus albus | D-glucose + cellotriose + cellobiose | - |
ir | |
| cellotriose + H2O | - |
Ruminococcus albus | D-glucose + cellobiose | - |
ir | |
| cellulose + H2O | ball-milled | Ruminococcus albus | D-glucose + ? | - |
? | |
| p-nitrophenyl-beta-D-cellobioside + H2O | - |
Ruminococcus albus | cellobiose + p-nitrophenol | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha,beta, 2 * 100000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Ruminococcus albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below 30°C | Ruminococcus albus |
| 37 | - |
20% activity loss after 10 min, 75% activity loss after 15 h at 37°C | Ruminococcus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Ruminococcus albus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Ruminococcus albus |
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