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Literature summary for 3.2.1.74 extracted from

  • Zhang, S.; Irwin, D.C.; Wilson, D.B.
    Site-directed mutation of noncatalytic residues of Thermobifida fusca exocellulase Cel6B (2000), Eur. J. Biochem., 267, 3101-3115.
    View publication on PubMed
Search for more literature for 3.2.1.74

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli and Streptomyces lividans Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
A229V loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
E495D active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
E495N active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G234A loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G234D loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G234N loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G234S loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose Thermobifida fusca
G234S/G284P loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside Thermobifida fusca
G284A loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G284I loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G284P loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G284P/A229V loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G331A active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
G496P loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
H326A active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
H326S active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose Thermobifida fusca
L230C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
L230C/D512C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
N233C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
N233C/D506C loop mutant, weaker binding affinities to all tested ligands Thermobifida fusca
W329C active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
W332A active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca
W332Y active site mutant, weaker binding affinities to all tested ligands Thermobifida fusca

Inhibitors

Inhibitors Comment Organism Structure
D-cellobiose competitive inhibition Thermobifida fusca

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermobifida fusca
-
-

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Thermobifida fusca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
-
 Thermobifida fusca ?
-
 ?
4-methylumbelliferyl beta-cellobioside + H2O
-
 Thermobifida fusca 4-methylumbelliferone + cellobiose
-
 ?
4-methylumbelliferyl beta-cellotrioside + H2O
-
 Thermobifida fusca 4-methylumbelliferone + cellotriose
-
 ?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
 Thermobifida fusca 4-methylumbelliferone + D-glucopyranose
-
 ?
carboxymethyl cellulose + H2O
-
 Thermobifida fusca ?
-
 ?
cellopentaose + H2O
-
 Thermobifida fusca D-glucose + ?
-
 ?
cellotetraose + H2O
-
 Thermobifida fusca D-glucose + cellotriose + cellobiose
-
 ?
cellotriose + H2O
-
 Thermobifida fusca D-glucose + cellobiose
-
 ?
cellulose + H2O H3PO4 swollen Thermobifida fusca D-glucose + ?
-
 ?
cellulose + H2O bacterial microcrystalline cellulose Thermobifida fusca D-glucose + ?
-
 ?