Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation to 132.1% at 5 mM | Aspergillus fumigatus | |
DMSO | activation to 111.5% at 5 mM | Aspergillus fumigatus | |
DTT | activation to 165.2% at 5 mM | Aspergillus fumigatus | |
EDTA | activation to 108.1% at 5 mM | Aspergillus fumigatus | |
L-cysteine | activation to 152.2% at 5 mM | Aspergillus fumigatus | |
Tween 20 | activation to 106.2% at 5 mM | Aspergillus fumigatus |
Application | Comment | Organism |
---|---|---|
additional information | upon addition to a commercial cellulase cocktail, Af-EGL7 significantly improves sugarcane exploded bagasse (SEB) saccharification, which suggests that enzyme Af-EGL7 has great potential to hydrolyze complex biomass. Af-EGL7 is a promising candidate to enhance enzyme cocktails used in biorefineries such as consolidated bioprocessing | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
gene AFUA_6G01800 or Af-egl7, DNA and amino acid sequnce determination and analysis, sequence comparisons, quantitative RT-PCR exoression analysis, recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS in inclusion bodies, subcloning in Escherichia coli strain DH10beta | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 5 mM | Aspergillus fumigatus | |
Cu2+ | 32.4% inhibition at 5 mM | Aspergillus fumigatus | |
Mn2+ | activates by 233% at 5 mM, best at 10 mM, slightly inhibitory at 20 mM | Aspergillus fumigatus | |
SDS | complete inhibition | Aspergillus fumigatus | |
Zn2+ | 20.4% inhibition at 5 mM | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for carboxymethyl cellulose is 209.7 mg/ml and for barley beta-glucan 113.9 mg/ml in presence of 5 mM Mn2+ | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | N-terminal signal peptide at residues 1-20 (MDSKRGVVAAVLALLPLVSA), enzyme Af-EGL7 is secreted | Aspergillus fumigatus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | activation to 105.5% at 5 mM | Aspergillus fumigatus | |
Ca2+ | activation to 135.7% at 5 mM | Aspergillus fumigatus | |
Co2+ | activation to 161.1% at 5 mM | Aspergillus fumigatus | |
Fe2+ | activation to 128.1% at 5 mM | Aspergillus fumigatus | |
KCl | activation to 110.4% at 5 mM | Aspergillus fumigatus | |
Mg2+ | activation to 122.6% at 5 mM | Aspergillus fumigatus | |
Mn2+ | activates by 233%, best at 10 mM, slightly inhibitory at 20 mM | Aspergillus fumigatus | |
NaCl | activation to 113.2% at 5 mM | Aspergillus fumigatus | |
Ni2+ | activation to 114.2% at 5 mM | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WCM9 | i.e. Aspergillus fumigatus | - |
Aspergillus fumigatus Af293 | Q4WCM9 | i.e. Aspergillus fumigatus | - |
Aspergillus fumigatus ATCC MYA-4609 | Q4WCM9 | i.e. Aspergillus fumigatus | - |
Aspergillus fumigatus CBS 101355 | Q4WCM9 | i.e. Aspergillus fumigatus | - |
Aspergillus fumigatus FGSC A1100 | Q4WCM9 | i.e. Aspergillus fumigatus | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has four potential N-glycosylation sites (N96, N201, N205, and N379) that are highly conserved among GH7 endoglucanases from other Aspergillus species | Aspergillus fumigatus |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies by denaturing and dialysis against Tris 10 mM, TCEP 0.1 mM, 0.05 mM EDTA, and 0.06% v/v N-lauroylsarcosine, pH 8.0, and protein refolding in 50 mM Tris, 12.5 mM cyclodextrin, and 1 mM EDTA, pH 7.5 by dialysis | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | best substrate, 1% w/v substrate concentration, best substrate | Aspergillus fumigatus | ? | - |
? | |
barley beta-glucan + H2O | best substrate, 1% w/v substrate concentration, best substrate | Aspergillus fumigatus CBS 101355 | ? | - |
? | |
barley beta-glucan + H2O | best substrate, 1% w/v substrate concentration, best substrate | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
? | |
barley beta-glucan + H2O | best substrate, 1% w/v substrate concentration, best substrate | Aspergillus fumigatus FGSC A1100 | ? | - |
? | |
barley beta-glucan + H2O | best substrate, 1% w/v substrate concentration, best substrate | Aspergillus fumigatus Af293 | ? | - |
? | |
carboxymethyl cellulose + H2O | 1% w/v substrate concentration, low activity | Aspergillus fumigatus | ? | - |
? | |
carboxymethyl cellulose + H2O | 1% w/v substrate concentration, low activity | Aspergillus fumigatus CBS 101355 | ? | - |
? | |
carboxymethyl cellulose + H2O | 1% w/v substrate concentration, low activity | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
? | |
carboxymethyl cellulose + H2O | 1% w/v substrate concentration, low activity | Aspergillus fumigatus FGSC A1100 | ? | - |
? | |
carboxymethyl cellulose + H2O | 1% w/v substrate concentration, low activity | Aspergillus fumigatus Af293 | ? | - |
? | |
additional information | the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan | Aspergillus fumigatus | ? | - |
? | |
additional information | the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan | Aspergillus fumigatus CBS 101355 | ? | - |
? | |
additional information | the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
? | |
additional information | the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan | Aspergillus fumigatus FGSC A1100 | ? | - |
? | |
additional information | the enzyme exhibits higher activity toward beta-glucan than toward xyloglucan and carboxymethyl cellulose suggesting that the enzyme corresponds to a beta-1,3-1,4-glucanase. No activity with Avicel and beechwood xylan | Aspergillus fumigatus Af293 | ? | - |
? | |
sugarcane exploded bagasse + H2O | 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin | Aspergillus fumigatus | ? | - |
? | |
sugarcane exploded bagasse + H2O | 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin | Aspergillus fumigatus CBS 101355 | ? | - |
? | |
sugarcane exploded bagasse + H2O | 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
? | |
sugarcane exploded bagasse + H2O | 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin | Aspergillus fumigatus FGSC A1100 | ? | - |
? | |
sugarcane exploded bagasse + H2O | 1% w/v substrate concentration, sugarcane exploded bagasse biomass (SEB). SEB is a highly heterogeneous material that consists of 47.5% cellulose, 9.0% hemicellulose, and 34.3% lignin | Aspergillus fumigatus Af293 | ? | - |
? | |
xyloglucan + H2O | high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration | Aspergillus fumigatus | ? | - |
? | |
xyloglucan + H2O | high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration | Aspergillus fumigatus CBS 101355 | ? | - |
? | |
xyloglucan + H2O | high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
? | |
xyloglucan + H2O | high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration | Aspergillus fumigatus FGSC A1100 | ? | - |
? | |
xyloglucan + H2O | high activity, xyloglucan from tamarind seed, 1% w/v substrate concentration | Aspergillus fumigatus Af293 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant enzyme, SDS-PAGE, x * 48000, about, sequence calculation | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
Af-EGL7 | - |
Aspergillus fumigatus |
Afu6g01800 | - |
Aspergillus fumigatus |
beta-1,3-1,4-glucanase | - |
Aspergillus fumigatus |
GH7 endo-1,4-beta-glucanase | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus fumigatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 40% of maximal activity at 30°C, 80% at 45°C and 60°C, about 35% at 70°C, and 20% at 80°C, profile overview | Aspergillus fumigatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | completely stable up to 50°C for 1 h and loss of 10% activity after 48 h at 50°C, 50% activity remaing at 55°C after 30 min and inactivation after 5 h at 55°C, rapid inactivation at 60°C | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43.32 | - |
carboxymethyl cellulose | pH 5.0, 55°C, recombinant enzyme | Aspergillus fumigatus | |
159.9 | - |
Barley beta-glucan | pH 5.0, 55°C, recombinant enzyme | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus fumigatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | activity range, over 50% of maximal activity at pH 4.0-5.5, profile overview | Aspergillus fumigatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | completely stable up to pH 6.0, 40% activity remaining at pH 7.0, inactivation at pH 8.0 | Aspergillus fumigatus |
Organism | Comment | Expression |
---|---|---|
Aspergillus fumigatus | substrate sugarcane exploded bagasse (SEB), induces Af-egl7 by 2500fold | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 7, GH7 | Aspergillus fumigatus |
additional information | residues 424-460 from the carbohydrate-binding domain, CBM1 domain | Aspergillus fumigatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for carboxymethyl cellulose is 0.207 mg/ml/s and for barley beta-glucan 1.404 mg/ml/s in presence of 5 mM Mn2+ | Aspergillus fumigatus |