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Literature summary for 3.2.1.73 extracted from
- Characterization of a lichenase isolated from soil metagenome (2014), J. Microbiol. Biotechnol., 24, 1699-1706.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
uncultured microorganism |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mt-lic, genomic library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, recombinant expression in Escherichia coli strain C43(DE3) | uncultured microorganism |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
uncultured microorganism | |
| additional information | poor inhibition by Fe3+ | uncultured microorganism | |
| Zn2+ | - |
uncultured microorganism | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 0.45 mg/ml with lichenan at pH 6.0, 50°C | uncultured microorganism |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme contains a signal peptide | uncultured microorganism | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates slightly | uncultured microorganism | |
| Fe2+ | activates | uncultured microorganism | |
| Mg2+ | activates | uncultured microorganism | |
| Mn2+ | activates slightly | uncultured microorganism | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41694 | - |
x * 45133, sequence calculation, x * 41694, recombinant enzyme without signal peptide, SDS-PAGE | uncultured microorganism |
| 45133 | - |
x * 45133, sequence calculation, x * 41694, recombinant enzyme without signal peptide, SDS-PAGE | uncultured microorganism |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley-beta-glucan + H2O | uncultured microorganism | - |
? | - |
? | |
| lichenan + H2O | uncultured microorganism | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured microorganism | KM079629 | isolated from soil metagenome, gene mt-lic | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain C43(DE3), the signal peptide is cleaved | uncultured microorganism |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.83 | - |
substrate lichenan, pH 6.0, 50°C | uncultured microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley-beta-glucan + H2O | - |
uncultured microorganism | ? | - |
? | |
| barley-beta-glucan + H2O | best substrate, azo-barley-glucan as the substrate | uncultured microorganism | ? | - |
? | |
| CM-cellulose + H2O | low activity | uncultured microorganism | ? | - |
? | |
| lichenan + H2O | - |
uncultured microorganism | ? | - |
? | |
| soluble starch + H2O | low activity | uncultured microorganism | ? | - |
? | |
| xylan + H2O | low activity | uncultured microorganism | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45133, sequence calculation, x * 41694, recombinant enzyme without signal peptide, SDS-PAGE | uncultured microorganism |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,3-1,4-glucanase | - |
uncultured microorganism |
| Lichenase | - |
uncultured microorganism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
uncultured microorganism |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range, profile overview | uncultured microorganism |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
uncultured microorganism |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | activity range, no activity below or above | uncultured microorganism |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 8 containing the amino acid sequence ATDGDLDIAYALLLASLQW | uncultured microorganism |
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